Anti-ERG 抗体 [EPR3864(2)] (ab133264)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3864(2)] to ERG
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-ERG antibody [EPR3864(2)]
ERG 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3864(2)] to ERG -
由来種
Rabbit -
特異性
This antibody does not cross-react with Fli1. -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Synthetic peptide within Human ERG aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P11308 -
ポジティブ・コントロール
- Jurkat whole cell lysate (ab7899), 293T cell lysate, MCF7 cell lysate, ERG recombinant protein, Human prostate adenocarcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 3.98 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3864(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/120.
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ICC/IF |
1/500.
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WB |
1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
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IP |
1/30.
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IHC-P | (3) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/120. |
ICC/IF
1/500. |
WB
1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). |
IP
1/30. |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. -
関連疾患
Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 2078 Human
- Entrez Gene: 13876 Mouse
- Omim: 165080 Human
- SwissProt: P11308 Human
- SwissProt: P81270 Mouse
- Unigene: 473819 Human
- Unigene: 164531 Mouse
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別名
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
see all
画像
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ab133264 staining ERGin the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/210. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/2000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human colonic carcinoma with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of MCF7 cells with purified ab133264 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133264 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.
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ab133264 (purified) at 1/30 immunoprecipitating ERG in HEK293 cells (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 54 kDa -
All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)
Lane 1 : Fli1 recombinant protein
Lane 2 : ERG recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 54 kDa -
Immunohistochemical analysis of paraffin embedded human prostate adenocarcinoma tissue labelling ERG with ab133264 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab133264 は 29 報の論文で使用されています。
- Rudnicki M et al. Quantitative Methods to Assess Adipose Vasculature. Methods Mol Biol 2441:201-221 (2022). PubMed: 35099739
- Gruber-Moesenbacher U et al. Myopericytoma arising from myopericytosis-a hitherto unrecognized entity within the lung. Virchows Arch 478:841-849 (2021). PubMed: 33244708
- Dong F et al. Dihydroartemisinin inhibits the expression of von Willebrand factor by downregulation of transcription factor ERG in endothelial cells. Fundam Clin Pharmacol 35:321-330 (2021). PubMed: 33107067
- Saulnier O et al. ERG transcription factors have a splicing regulatory function involving RBFOX2 that is altered in the EWS-FLI1 oncogenic fusion. Nucleic Acids Res 49:5038-5056 (2021). PubMed: 34009296
- Li L et al. Aberrant Activation of Notch1 Signaling in Glomerular Endothelium Induces Albuminuria. Circ Res 128:602-618 (2021). PubMed: 33435713