Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal liver metastasis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8
Permeabilization
No
Specification
Colorectal liver metastasis
Blocking step
TBS-0.01% Tween 20 + 10% normal horse serum + 1.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-0.01% Tween 20 + 1.5% BSA
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector Biotinylated Horse Anti-Rabbit IgG Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
The antibody failed to detect ERBB3 in our sample using the manufacturer's recommended protocol steps, despite having been used for similar samples in several publications. However, we we were able to detect our protein of interest in the same sample using polyclonal ab227775, which was kindly provided to us by abcam as part of their refund policy.
Abcam response
Thank you for your honest feedback. We are sorry to hear that the antibody did not work as expected. The antibody is tested in immunohistochemistry with paraffin embedded sections (IHC-P) and in human samples and we resolved this issue in accordance with our AbPromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 24 2018