Anti-ErbB3 / HER3 抗体 [E186] (ab32121)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E186] to ErbB3 / HER3
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ErbB3 / HER3 antibody [E186]
ErbB3 / HER3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E186] to ErbB3 / HER3 -
由来種
Rabbit -
特異性
ab32121 recognises ErbB3/HER3. WB samples: should avoid boiling -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC or IP -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide within Human ErbB3/ HER3 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21860 -
ポジティブ・コントロール
- MCF-7 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E186 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32121の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa). |
ターゲット情報
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機能
Binds and is activated by neuregulins and NTAK. -
組織特異性
Epithelial tissues and brain. -
関連疾患
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
発生段階
Overexpressed in a subset of human mammary tumors. -
ドメイン
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻訳後修飾
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2065 Human
- Entrez Gene: 29496 Rat
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q62799 Rat
- Unigene: 118681 Human
- Unigene: 10228 Rat
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別名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERBB3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. Ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa -
All lanes : Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/ml (purified)
Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Exposure time: 37 seconds -
Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate
Predicted band size: 149 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab32121 は 18 報の論文で使用されています。
- Dan W et al. RIP1-dependent Apoptosis and Differentiation Regulated by Skp2 and Akt/GSK3β in Acute Myeloid Leukemia. Int J Med Sci 19:525-536 (2022). PubMed: 35370472
- Zhang M et al. LncRNA AK020546 protects against cardiac ischemia-reperfusion injury by sponging miR-350-3p. Aging (Albany NY) 13:14219-14233 (2021). PubMed: 33988127
- Bai S et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
- Zhu J et al. Role of the Nrdp1 in Brain Injury Induced by Chronic Intermittent Hypoxia in Rats via Regulating the Protein Levels of ErbB3. Neurotox Res 38:124-132 (2020). PubMed: 32200526
- Pereira PMR et al. HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer. Mol Pharm 17:327-337 (2020). PubMed: 31804840