Anti-ErbB2 / HER2 抗体 [ICR12] (ab11710)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.co.jp/ps/products/11/ab11710/Images/ab11710-1-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [ICR12] to ErbB2 / HER2
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ErbB2 / HER2 antibody [ICR12]
ErbB2 / HER2 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [ICR12] to ErbB2 / HER2 -
由来種
Rat -
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details
適用なし: IP or WB -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human breast carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt: SK-BR-3 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
ICR12 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| Flow Cyt |
1/1000.
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| 特記事項 |
|---|
|
IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
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Flow Cyt
1/1000. |
ターゲット情報
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機能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
組織特異性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
関連疾患
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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参照データベース
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Membranous staining on human breast carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.co.jp/ps/products/11/ab11710/Images/ab11710-3-anti-erbb-2-antibody-icr12-immunocytochemistry-skbr3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 cells labelling ErbB 2 with ab11710 at 1/100 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous staining in SK-BR-3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 diliution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab11710 at 1/100 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Negative control cells: MCF7 (PMID: 18288420).
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![Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.co.jp/ps/products/11/ab11710/Images/ab11710-4-anti-erbb-2-antibody-icr12-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left panel) / SK-BR-3 (human breast adenocarcinoma epithelial cell, Right panel) cells labelling ErbB 2 with ab11710 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody.
Low expression control: MCF7 (PMID: 17938260).
Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.co.jp/ps/products/11/ab11710/Images/ab11710-2-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB 2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). No staining on human breast carcinoma without expression of HER2 is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)](https://www.abcam.co.jp/ps/products/11/ab11710/Images/ab11710-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [ICR12] (ab11710)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab11710 は 3 報の論文で使用されています。
- Zheng G et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther 6:236 (2021). PubMed: 34158475
- Smellie WJ et al. Radioimmunotherapy of breast cancer xenografts with monoclonal antibody ICR12 against c-erbB2 p185: comparison of iodogen and N-succinimidyl 4-methyl-3-(tri-n-butylstannyl)benzoate radioiodination methods. Cancer Res 55:5842s-5846s (1995). PubMed: 7493357
- Eccles SA et al. Regression of established breast carcinoma xenografts with antibody-directed enzyme prodrug therapy against c-erbB2 p185. Cancer Res 54:5171-7 (1994). PubMed: 7923136
