Anti-ErbB2 / HER2 抗体 [9G6] (ab16899)
![Flow Cytometry - Anti-ErbB2 / HER2 antibody [9G6] (ab16899)](https://www.abcam.co.jp/ps/products/16/ab16899/Images/ab16899-195311-anti-erbb-2-antibody-9g6-flow-cytometry.jpg "Flow Cytometry - Anti-ErbB2 / HER2 antibody [9G6] (ab16899)")
Key features and details
- Mouse monoclonal [9G6] to ErbB2 / HER2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ErbB2 / HER2 antibody [9G6]
ErbB2 / HER2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9G6] to ErbB2 / HER2 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog
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免疫原
Synthetic peptide corresponding to Human ErbB2/ HER2 (C terminal).
Database link: P04626 -
ポジティブ･コントロール
- SK-BR-3 cells or breast carcinoma tissue
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特記事項
Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.2% Gelatin, 0.82% Sodium phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
9G6 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Affibody® Molecule
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
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機能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
組織特異性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
関連疾患
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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参照データベース
- Entrez Gene: 403883 Dog
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: O18735 Dog
- SwissProt: P04626 Human
- Unigene: 446352 Human
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別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
画像
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Flow Cytometry - Anti-ErbB2 / HER2 antibody [9G6] (ab16899)Overlay histogram showing MCF7 cells stained with ab16899 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16899, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab16899 は 6 報の論文で使用されています。
- Kapara A et al. Detection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS. Anal Chem 93:5862-5871 (2021). PubMed: 33797884
- Al-Zeheimi N & Adham SA Modeling Neoadjuvant chemotherapy resistance in vitro increased NRP-1 and HER2 expression and converted MCF7 breast cancer subtype. Br J Pharmacol 177:2024-2041 (2020). PubMed: 31883395
- Pietilä M et al. SORLA regulates endosomal trafficking and oncogenic fitness of HER2. Nat Commun 10:2340 (2019). PubMed: 31138794
- Bagdany M et al. Chaperones rescue the energetic landscape of mutant CFTR at single molecule and in cell. Nat Commun 8:398 (2017). PubMed: 28855508
- Gomes de Castro MA et al. Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. PLoS One 12:e0173050 (2017). PubMed: 28235049
- van de Vijver MJ et al. Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer. N Engl J Med 319:1239-45 (1988). IHC-P . PubMed: 2903446