Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland from Her2-positive mouse)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
Yes - Triton X-100 0.2% for 30 min
Specification
Mammary gland from Her2-positive mouse
Blocking step
Mouse IgG blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 90µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 25°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse Ig Impress
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
For a mouse sample, as I used, it's necessary to use a mouse-on mouse kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Flavia Ghiraldini
Verified customer
投稿 Apr 11 2017