Anti-EPS8L1 抗体 (ab272642)
Key features and details
- Rabbit polyclonal to EPS8L1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EPS8L1 antibody -
製品の詳細
Rabbit polyclonal to EPS8L1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human EPS8L1 aa 350-500.
Database link: Q8TE68 -
ポジティブ･コントロール
- WB: RT4 cells. IHC-P: Human placenta tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. -
組織特異性
Detected in placenta. -
配列類似性
Belongs to the EPS8 family.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 54869 Human
- Omim: 614987 Human
- SwissProt: Q8TE68 Human
- Unigene: 438862 Human
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別名
- DRC3 antibody
- Epidermal growth factor receptor kinase substrate 8 like protein 1 antibody
- Epidermal growth factor receptor kinase substrate 8-like protein 1 antibody
see all
画像
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Western blot - Anti-EPS8L1 antibody (ab272642)Lane 2 : Anti-EPS8L1 antibody (ab272642) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS8L1 antibody (ab272642)Paraffin-embedded formalin-fixed human placenta tissue stained for EPS8L1 using ab272642 at 1/200 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272642 は論文での使用が確認できていません。