Application
Flow Cytometry
Sample
Mouse Cell (small cell lung cancer cell line 16T)
Permeabilization
No
Gating Strategy
single cells with FSC and SSC, live cells with low DAPI, positive/negative cells with PI filter set, anti-CD24 as positive control
Specification
small cell lung cancer cell line 16T
Preparation
Cell harvesting/tissue preparation method: Cell harvesting: cells grow as floating spheres. Collect by spinning, dissociate spheres with TrypLE, single cell through 40um cell strainer
Sample buffer: PBS + 10% FBS
Sample buffer: PBS + 10% FBS
Fixation
none
Other product details
Incubation time
30 minute(s) · Temperature: 0°C · Diluent: PBS + 10% FBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
Thank you for submitting your feedback. We have not validated this antibody for use in Flow Cytometry and will continue to gather data on use in this application. We appreciate the sharing of your data with us and other researchers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 26 2018