Anti-Ephrin B3 抗体 (ab101699)
Key features and details
- Rabbit polyclonal to Ephrin B3
- Suitable for: WB, IHC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Ephrin B3 antibody
Ephrin B3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ephrin B3 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
アプリケーション
適用あり: WB, IHCmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Cow, Cat, Dog, Pig, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human Ephrin B3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab113310) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa).
|
|
IHC |
Use at an assay dependent concentration. PubMed: 25309991
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa). |
IHC
Use at an assay dependent concentration. PubMed: 25309991 |
ターゲット情報
-
機能
May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. -
組織特異性
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments. -
配列類似性
Belongs to the ephrin family. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1949 Human
- Entrez Gene: 13643 Mouse
- Entrez Gene: 360546 Rat
- Omim: 602297 Human
- SwissProt: Q15768 Human
- SwissProt: O35393 Mouse
- Unigene: 26988 Human
- Unigene: 249637 Mouse
-
別名
- EFL 6 antibody
- EFL6 antibody
- EFNB 3 antibody
see all
画像
-
All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lane 5 : Rat Forebrain
Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
Lane 7 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
Ephrin B3 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab101699 は 4 報の論文で使用されています。
- Stepan J et al. Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning. Cell Rep 41:111766 (2022). PubMed: 36476872
- Xiong Y et al. Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury. Mol Med Rep 18:2171-2181 (2018). PubMed: 29901151
- Efazat G et al. Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer. Oncotarget 7:60332-60347 (2016). PubMed: 27533087
- Qu B et al. Testing stem cell therapy in a rat model of inflammatory bowel disease: role of bone marrow stem cells and stem cell factor in mucosal regeneration. PLoS One 9:e107891 (2014). IHC ; Rat . PubMed: 25309991