Anti-Ephrin B2 抗体 (ab131536)
Key features and details
- Rabbit polyclonal to Ephrin B2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Ephrin B2 antibody
Ephrin B2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ephrin B2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Ephrin B2 aa 328-332 conjugated to keyhole limpet haemocyanin.
Sequence:N-I-Y-Y-K
Database link: P52799 -
ポジティブ・コントロール
- HEK293, 293T, PC3, A549, Hela, 293, MCF, and Mouse and Rat Spleen and Brain tissue lysate; 293 cells treated with 20ng/ml TNF-a for 30min.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131536の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/200 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa).
Predicted molecular weight: 37 kDa. Observed molecular weight: 55 kDa. The observed band size of Ephrin-B2 may not the same as predicted MWs in WB (PMID: 31985015,32286515). |
特記事項 |
---|
WB
1/200 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa). Predicted molecular weight: 37 kDa. Observed molecular weight: 55 kDa. The observed band size of Ephrin-B2 may not the same as predicted MWs in WB (PMID: 31985015,32286515). |
ターゲット情報
-
機能
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. -
組織特異性
Lung and kidney. -
配列類似性
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain. -
翻訳後修飾
Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1948 Human
- Entrez Gene: 13642 Mouse
- Entrez Gene: 306636 Rat
- Omim: 600527 Human
- SwissProt: P52799 Human
- SwissProt: P52800 Mouse
- Unigene: 149239 Human
- Unigene: 209813 Mouse
-
別名
- EFN B2 antibody
- EFNB 2 antibody
- Efnb2 antibody
see all
画像
-
Anti-Ephrin B2 antibody (ab131536) at 1/800 dilution + A549 cell lysate at 40 µg
Secondary
HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution
Predicted band size: 37 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
All lanes : Anti-Ephrin B2 antibody (ab131536) at 1/2000 dilution
Lane 1 : Mouse Spleen tissue lysate
Lane 2 : Rat Spleen tissue lysate
Lane 3 : HEK293 tissue lysate
Lane 4 : 293T tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG(HRP) at 1/5000 dilution
Predicted band size: 37 kDa -
All lanes : Anti-Ephrin B2 antibody (ab131536) at 1/800 dilution
Lane 1 : PC3 tissue lysate
Lane 2 : A549 tissue lysate
Lane 3 : Hela tissue lysate
Lane 4 : 293 tissue lysate
Lane 5 : MCF tissue lysate
Lane 6 : Mouse Spleen tissue lysate
Lane 7 : Rat Brain tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG(HRP) at 1/5000 dilution
Predicted band size: 37 kDa -
Anti-Ephrin B2 antibody (ab131536) at 1/200 dilution + Mouse brain tissue lysate at 40 µg
Secondary
Goat anti rabbit IgG at 1/8000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsGel: 8%SDS-PAGE
-
All lanes : Anti-Ephrin B2 antibody (ab131536) at 1/1000 dilution
Lane 1 : 293 cells treated with 20ng/ml TNF-a for 30min
Lane 2 : 293 cells blocked with the phospho protein
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (18)
ab131536 は 18 報の論文で使用されています。
- Kakarla M et al. Ephrin B Activate Src Family Kinases in Fibroblasts Inducing Stromal Remodeling in Prostate Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35565468
- VanderWeele DJ et al. A Phase II Study of sEphB4-HSA in Metastatic Castration-Resistant Prostate Cancer. Clin Genitourin Cancer 20:575-580 (2022). PubMed: 36210299
- Wolf KG et al. Ephrin-B2-expressing natural killer cells induce angiogenesis. JVS Vasc Sci 3:336-344 (2022). PubMed: 36439700
- Jiang Y et al. Development of an arteriolar niche and self-renewal of breast cancer stem cells by lysophosphatidic acid/protein kinase D signaling. Commun Biol 4:780 (2021). PubMed: 34168243
- Park ES et al. Selective Endothelial Hyperactivation of Oncogenic KRAS Induces Brain Arteriovenous Malformations in Mice. Ann Neurol 89:926-941 (2021). PubMed: 33675084