Anti-Eph receptor B4/HTK 抗体 (ab93902)
Key features and details
- Rabbit polyclonal to Eph receptor B4/HTK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Eph receptor B4/HTK antibody
Eph receptor B4/HTK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Eph receptor B4/HTK -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Monkey -
免疫原
Synthetic peptide corresponding to Human Eph receptor B4/HTK aa 850-950 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107107) -
ポジティブ・コントロール
- This antibody gave a positive signal in both Human placenta tissue lysate and A549 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product. |
ターゲット情報
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機能
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
組織特異性
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 515756 Cow
- Entrez Gene: 2050 Human
- Entrez Gene: 13846 Mouse
- Omim: 600011 Human
- SwissProt: P54760 Human
- SwissProt: P54761 Mouse
- Unigene: 437008 Human
- Unigene: 34533 Mouse
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別名
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
画像
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All lanes : Anti-Eph receptor B4/HTK antibody (ab93902) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThe predicted molecular weight of Eph receptor B4/HTK is 108 kDa (SwissProt), however we expect to observe a banding pattern at 125 kDa. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab93902 は論文での使用が確認できていません。