Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6457] to Eph receptor B1/NET - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Eph receptor B1/NET antibody [EPR6457] - BSA and Azide free
Eph receptor B1/NET 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6457] to Eph receptor B1/NET - BSA and Azide free
Customer data indicates that this antibody reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Eph receptor B1/NET aa 550-650 (phospho Y594). The exact sequence is proprietary.
Ab248301 is the carrier-free version of ab129103. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248301 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 2.13 x 10 -10 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
機能Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
組織特異性Preferentially expressed in brain.
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- Cek 6 antibody
- EK6 antibody
- ELK antibody
All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : COS1 cell lysate
Lane 5 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
This data was developed using ab129103, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.