Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Retrieval Buffer, microwave
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/250
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% serum
Secondary antibody
Name
Non-Abcam antibody was used: Vector ABC Kit PK-6200
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Antigen retrieval with heat (microwave) required. ABC-amplification, Nova-Red substrate (Vector Laboratories).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Eva Schmelzer
Verified customer
投稿 Jul 30 2010