Application
Flow Cytometry
Sample
Human Cell (breast cancer cells)
Specification
breast cancer cells
Permeabilization
No
Other product details
Concentration
1 µg/cells
Incubation time
6 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
It was a FACs experiments: cells were incubated with 0,5 mM of inmuno-liposomes created with the EpCam antiboody for 6 hours. You can find fluorescence only in the cells expressing EpCAM (MG1). The concentration of the antibody is aproximated because it´s quite difficult to measure it in the inmuno-liposomes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Enrique Barrajon
Verified customer
投稿 Dec 11 2006