Key features and details
- Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM (FITC)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
製品名Anti-EpCAM antibody [B29.1 (VU-1D9)] (FITC)
EpCAM 一次抗体 製品一覧
製品の詳細Mouse monoclonal [B29.1 (VU-1D9)] to EpCAM (FITC)
標識FITC. Ex: 493nm, Em: 528nm
特異性This antibody reacts with a 40 kD cell surface glycoprotein called ESA.
アプリケーション適用あり: WB, IP, ICC/IFmore details
Tissue, cells or virus corresponding to Human EpCAM. Raised against carcinoma cell line of human origin.
Database link: P16422
- Breast, colon carcinoma and tonsil
Dilute according to the particular application being used. In general, 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Fluorescein (FITC) conjugated at a ratio of 6 moles of Fluorescein to 1 mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm and maximum emission occurs at wavelength 520 nm.
保存方法Shipped at 4°C. Store at +4°C. Please see notes section.
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab8666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.
This product was not quality controlled in Flow Cytometry applications. However, it may be applicable for Flow Cytometry at a concentration of 1-2 µg in 0.1 ml containing 10^6 cells.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. See Abreview.|
機能May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
組織特異性Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
関連疾患Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
配列類似性Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain.
翻訳後修飾Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
細胞内局在Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
- Information by UniProt
- 17 1A antibody
- 323/A3 antibody
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ICC/IF image of ab8666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab8666 は 14 報の論文で使用されています。
- Cheng Z et al. Long non-coding RNA THOR promotes liver cancer stem cells expansion via ß-catenin pathway. Gene 684:95-103 (2019). PubMed: 30359743
- Ran RZ et al. miR-194 inhibits liver cancer stem cell expansion by regulating RAC1 pathway. Exp Cell Res 378:66-75 (2019). PubMed: 30844391
- Jiang ZB et al. miR-365 regulates liver cancer stem cells via RAC1 pathway. Mol Carcinog 58:55-65 (2019). PubMed: 30182377
- Zhang P et al. Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer. Cell Rep 27:1934-1947.e5 (2019). PubMed: 31067475
- Parker SG et al. A photoelectrochemical platform for the capture and release of rare single cells. Nat Commun 9:2288 (2018). PubMed: 29895867