Application
Western blot
Sample
Rabbit Cell lysate - whole cell (pancreatic acini)
Loading amount
20 µg
Specification
pancreatic acini
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 5 %
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
supersignal
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 99 kDa
Positive control
kidney, brain, pancreas, pancretic acini
Negative control
liver,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. maria sabbatini
Verified customer
投稿 Sep 09 2008