Anti-ENTPD5 抗体 [EPR3784] (ab108603)
![Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.co.jp/ps/products/108/ab108603/Images/ab108603-121320-anti-entpd5-antibody-epr3784-western-blot.gif "Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3784] to ENTPD5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ENTPD5 antibody [EPR3784]
ENTPD5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3784] to ENTPD5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab190069) -
ポジティブ･コントロール
- Fetal liver cell lysate; Human kidney tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR3784 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108603の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
追加情報
Is unsuitable for Flow Cyt or ICC.
ターゲット情報
-
機能
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. -
組織特異性
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the GDA1/CD39 NTPase family. -
翻訳後修飾
N-glycosylated; high-mannose type. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
-
参照データベース
- Entrez Gene: 957 Human
- Entrez Gene: 12499 Mouse
- Entrez Gene: 314312 Rat
- Omim: 603162 Human
- SwissProt: O75356 Human
- SwissProt: Q9WUZ9 Mouse
- SwissProt: Q6P6S9 Rat
- Unigene: 720540 Human
see all -
別名
- CD39 like 4 antibody
- AI196558 antibody
- AI987697 antibody
see all
画像
-
Western blot - Anti-ENTPD5 antibody [EPR3784] (ab108603)Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.co.jp/ps/products/108/ab108603/Images/ab108603-121319-anti-entpd5-antibody-epr3784-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENTPD5 antibody [EPR3784] (ab108603)ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-ENTPD5 antibody [EPR3784] (ab108603)](https://www.abcam.co.jp/ps/products/108/ab108603/Images/ab108603-3-benefits-of-recombinant-antibodies.png)
Anti-ENTPD5 antibody [EPR3784] (ab108603)
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (4)
ab108603 は 4 報の論文で使用されています。
- Wei J et al. ER-associated ubiquitin ligase HRD1 programs liver metabolism by targeting multiple metabolic enzymes. Nat Commun 9:3659 (2018). PubMed: 30201971
- Shah S et al. Targeting ACLY sensitizes castration-resistant prostate cancer cells to AR antagonism by impinging on an ACLY-AMPK-AR feedback mechanism. Oncotarget 7:43713-43730 (2016). PubMed: 27248322
- Walsh J et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . PubMed: 24859727
- Curry NL et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov 3:908-21 (2013). WB, IHC-P ; Mouse . PubMed: 23719831
