Anti-Entactin/NID 抗体 - BSA and Azide free (Capture) (ab272277)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to Entactin/NID - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
製品の概要
-
製品名
Anti-Entactin/NID antibody - BSA and Azide free (Capture)
Entactin/NID 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal to Entactin/NID - BSA and Azide free (Capture) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Mouse -
特記事項
ab272277 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Nidogen-1. The recommended pair for sandwich ELISA is:
Capture: ab272277, Mouse Nidogen-1 Capture Antibody (unconjugated)
Detector: ab272278, Mouse Nidogen-1 Detector Antibody (unconjugated)
The reference range value is 78.125 - 5000 pg/ml.The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
-
ポリ/モノ
モノクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
sELISA pair antibody
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
|
特記事項 |
---|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
ターゲット情報
-
機能
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
配列類似性
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 18073 Mouse
- SwissProt: P10493 Mouse
- Unigene: 4691 Mouse
-
別名
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272277 は論文での使用が確認できていません。