Application
Western blot
Sample
Cow Cell lysate - whole cell (Aortic Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
10 µg
Specification
Aortic Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Mouse melanoma B16F10
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 130 kDa
Positive control
Endothelial cells should be positive control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 19 2017