Anti-Engrailed and Invected 抗体 [4D9] (ab12454)
![IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)](https://www.abcam.co.jp/ps/products/12/ab12454/Images/ab12454-20051-anti-engrailed-and-invected-antibody-4d9-ihc-wholemount.jpg "IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)")
Key features and details
- Mouse monoclonal [4D9] to Engrailed and Invected
- Suitable for: IHC - Wholemount
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
製品の概要
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製品名
Anti-Engrailed and Invected antibody [4D9] -
製品の詳細
Mouse monoclonal [4D9] to Engrailed and Invected -
由来種
Mouse -
アプリケーション
適用あり: IHC - Wholemountmore details -
種交差性
交差種: Drosophila melanogaster
交差が予測される動物種: Chicken, Xenopus laevis, Zebrafish, Crayfish
非交差種:
Mouse -
免疫原
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
エピトープ
Epitope has been localized to residues 38-58 of the homeodomain. -
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
4D9 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC - Wholemount |
1/200.
Fix with 4% paraformaldehyde in PBS for 1 hour.
|
| 特記事項 |
|---|
|
IHC - Wholemount
1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.
|
ターゲット情報
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関連性
In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear. -
参照データベース
- Entrez Gene: 36239 Drosophila melanogaster
- Entrez Gene: 36240 Drosophila melanogaster
- SwissProt: P02836 Drosophila melanogaster
- SwissProt: P05527 Drosophila melanogaster
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別名
- En antibody
- Eng antibody
- Engrailed antibody
see all
画像
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IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.
ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available here.
Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.
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![IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)](https://www.abcam.co.jp/ps/products/12/ab12454/Images/ab12454-20050-anti-engrailed-and-invected-antibody-4d9-ihc-wholemount.jpg)
IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available here.
Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining.
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![IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)](https://www.abcam.co.jp/ps/products/12/ab12454/Images/ab12454-316020-anti-engrailed-and-invected-antibody-4d9-ihc-wholemount.jpg)
IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (ab12454)This image is courtesy of Dr Torsten Bossing at the University of Plymouth.ab12454 used in immunohistochemistry (Wholemount) at 1/200 dilution on Drosophila embryos. The protocol used is available here.
Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.
データシートおよび資料
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Datasheet download
参考文献 (12)
ab12454 は 12 報の論文で使用されています。
- Blaudin de Thé FX et al. Engrailed homeoprotein blocks degeneration in adult dopaminergic neurons through LINE-1 repression. EMBO J 37:N/A (2018). PubMed: 29941661
- Baratte S et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). PubMed: 17394016
- Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). PubMed: 12781681
- Nakamura Y et al. Identification of a Drosophila gene encoding xylosylprotein beta4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis. J Biol Chem 277:46280-8 (2002). PubMed: 12215432
- Nagaso H et al. Simultaneous detection of RNA and protein by in situ hybridization and immunological staining. J Histochem Cytochem 49:1177-82 (2001). PubMed: 11511686
