Anti-Endothelin 1 抗体 [TR.ET.48.5] (ab2786)
Key features and details
- Mouse monoclonal [TR.ET.48.5] to Endothelin 1
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Human, Pig
- Isotype: IgG1
製品の概要
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製品名
Anti-Endothelin 1 antibody [TR.ET.48.5]
Endothelin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TR.ET.48.5] to Endothelin 1 -
由来種
Mouse -
特異性
Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine). -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Human, Pig -
免疫原
Full length protein corresponding to Human Endothelin 1 conjugated to Keyhole Limpet Haemocyanin (KLH).
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エピトープ
Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16. -
ポジティブ・コントロール
- human bowel
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
TR.ET.48.5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | 1/200 - 1/1000. | |
IHC-P | 1/250. | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
ターゲット情報
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機能
Endothelins are endothelium-derived vasoconstrictor peptides. -
組織特異性
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. -
配列類似性
Belongs to the endothelin/sarafotoxin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1906 Human
- Entrez Gene: 13614 Mouse
- Entrez Gene: 396915 Pig
- Entrez Gene: 100009270 Rabbit
- Entrez Gene: 24323 Rat
- Entrez Gene: 443498 Sheep
- Omim: 131240 Human
- SwissProt: P05305 Human
see all -
別名
- ARCND3 antibody
- Big endothelin-1 antibody
- EDN1 antibody
see all
画像
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ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution + PC12 cell lysate at 25 µg
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786)Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.
プロトコール
参考文献 (39)
ab2786 は 39 報の論文で使用されています。
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- Zhai R et al. Exendin-4, a GLP-1 receptor agonist regulates retinal capillary tone and restores microvascular patency after ischaemia-reperfusion injury. Br J Pharmacol 177:3389-3402 (2020). PubMed: 32232832
- Zhang J et al. MicroRNA-483 amelioration of experimental pulmonary hypertension. EMBO Mol Med 12:e11303 (2020). PubMed: 32324970
- Liu SX et al. Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis. Mol Ther Nucleic Acids 18:34-44 (2019). PubMed: 31479923
- Wang H et al. Modulating effects of preconditioning exercise in the expression of ET-1 and BNP via HIF-1a in ischemically injured brain. Metab Brain Dis 34:1299-1311 (2019). PubMed: 31222402