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  1. Link

    endothelial-cell-antibody-reca-1-ab9774.pdf

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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
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Anti-Endothelial Cell 抗体 [RECA-1] (ab9774)

  • Datasheet
  • SDS
Reviews (18)Q&A (18)References (89)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (ab9774)

    Key features and details

    • Mouse monoclonal [RECA-1] to Endothelial Cell
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488

    こちらの製品もご検討ください

    IHC
    Normal Horse Serum (sterile) (ab139501)
    IHC
    Glycerol Mounting Medium - Anti-Fade with DAPI (ab188804)
    二次抗体
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    関連製品

    製品の概要

    • 製品名

      Anti-Endothelial Cell antibody [RECA-1]
      Endothelial Cell 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [RECA-1] to Endothelial Cell
    • 由来種

      Mouse
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Rat
    • 免疫原

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • ポジティブ・コントロール

      • Rat brain FFPE section (IHC)
    • 特記事項

      RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Concentration information loading...
    • 精製度

      Protein G purified
    • 一次抗体 備考

      RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
    • ポリ/モノ

      モノクローナル
    • クローン名

      RECA-1
    • アイソタイプ

      IgG1
    • 研究分野

      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial

    関連製品

    • Alternative Versions

      • Alexa Fluor® 488 Anti-Endothelial Cell antibody [RECA-1] (ab197727)
      • Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (ab264524)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9774の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (5)
    Use a concentration of 10 µg/ml.
    特記事項
    IHC-P
    Use a concentration of 10 µg/ml.

    ターゲット情報

    • 関連性

      The endothelium is a single layer of cells lining various organs and cavities of the body, especially the blood vessels, heart, and lymphatic vessels.Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (ab9774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (ab9774)
      IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (89)

    ab9774 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9774 は 89 報の論文で使用されています。

    • Wang P  et al. Melatonin ameliorates microvessel abnormalities in the cerebral cortex and hippocampus in a rat model of Alzheimer's disease. Neural Regen Res 16:757-764 (2021). PubMed: 33063739
    • Zhong Y  et al. Inhibiting Th1/2 cells influences hepatic capillarization by adjusting sinusoidal endothelial fenestrae through Rho-ROCK-myosin pathway. Aging (Albany NY) 13:5069-5086 (2021). PubMed: 33535174
    • Duan YY  et al. Microtubule Stabilization Promotes Microcirculation Reconstruction After Spinal Cord Injury. J Mol Neurosci 71:583-595 (2021). PubMed: 32901373
    • Uzunalli G  et al. Structural disruption of the blood-brain barrier in repetitive primary blast injury. Fluids Barriers CNS 18:2 (2021). PubMed: 33413513
    • Kútna V  et al. Tuberous Sclerosis (tsc2+/-) Model Eker Rats Reveals Extensive Neuronal Loss with Microglial Invasion and Vascular Remodeling Related to Brain Neoplasia. Neurotherapeutics 17:329-339 (2020). PubMed: 31820275
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 36 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.5% triton
    Specification
    Brain
    Blocking step
    Goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 09 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5%
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 27 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X100
    Specification
    Spinal cord
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4 · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    DR. CHI ZHANG

    Verified customer

    投稿 Apr 27 2018

    Immunohistochemistry free floating abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Rat striatum, endothelial cells)
    Specification
    Rat striatum, endothelial cells
    Read More

    DR. Edward Fletcher

    Verified customer

    投稿 Jul 13 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - triton 1%
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    MRS. Francoise Geffroy

    Verified customer

    投稿 Jan 11 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    DR. Fabio Marongiu

    Verified customer

    投稿 Oct 20 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain tissue)
    Antigen retrieval step
    None
    Specification
    Brain tissue
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10 · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Sumit Sarkar

    Verified customer

    投稿 Aug 14 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate pH=6
    Sample
    Rat Tissue sections (omentum)
    Specification
    omentum
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 09 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Endothelial Cell antibody [RECA-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Heart)
    Specification
    Heart
    Fixative
    10% Neutral Buffered Formalin, 24h
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 23 2012

    Question

    I wonder if you have the RECA-1 from RABBIT react on RAT.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 15 2012

    Answer

    Unfortunately this clone, RECA-1, for anti-Anti-Endothelial Cell antibody is produced only in mouse. I have reviewed other products on our catalog and it appears that none of our other Anti-Endothelial Cell antibodies are produced in rabbit either. I am sorry for the inconvenience this may cause.

    Read More

    Abcam Scientific Support

    Answered on Dec 15 2012

    1-10 of 36 Abreviews or Q&A

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