Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5406(B)] to ENC1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
製品名Anti-ENC1 antibody [EPR5406(B)] - BSA and Azide free
ENC1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5406(B)] to ENC1 - BSA and Azide free
アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
交差が予測される動物種: Mouse, Rat
Synthetic peptide within Human ENC1 aa 550-650. The exact sequence is proprietary.
Ab248050 is the carrier-free version of ab124902. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.03 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
機能Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
組織特異性Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.
パスウェイProtein modification; protein ubiquitination.
配列類似性Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
発生段階Dramatically up-regulated upon neuronal differentiation.
翻訳後修飾Ubiquitinated and probably targeted for proteasome-independent degradation.
細胞内局在Nucleus matrix. Cytoplasm > cytoskeleton.
- Information by UniProt
- CCL 28 antibody
- CCL28 antibody
- Ectoderm neural cortex protein 1 antibody
Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
This data was developed using ab124902, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.