Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5660] to ENAH/MENA - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Human
製品名Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free
ENAH/MENA 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5660] to ENAH/MENA - BSA and Azide free
アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
適用なし: ICC or IP
種交差性交差種: Mouse, Human
Synthetic peptide within Human ENAH/MENA aa 550-650. The exact sequence is proprietary.
Database link: Q8N8S7
ab247959 is the carrier-free version of ab124685 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ENAH
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|WB||Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).|
機能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
組織特異性Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
配列類似性Belongs to the Ena/VASP family.
Contains 1 WH1 domain.
ドメインThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
翻訳後修飾NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
- Information by UniProt
- ENA antibody
- Enah antibody
- ENAH_HUMAN antibody
All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution
Lane 1 : MDA-MB-435 (Human ductal carcinoma cell line) cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
This data was developed using ab124685, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.