Key features and details
- Rabbit polyclonal to EMSY
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
EMSY 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to EMSY
特異性This antibody detects a band of the same size by Western blot as ab4579, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years after this antibody). The band detected by this antibody also runs at the same height as full length in vitro translated EMSY. This confirms that the band detected is EMSY.
アプリケーション適用あり: WB, IPmore details
種交差性交差種: Mouse, Human
Recombinant fragment corresponding to Human EMSY aa 100-200.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa). Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD. However, the independently raised ab4579 has been used to confirm the specificity of this antibody.|
- Information by UniProt
- C11orf30 antibody
- Chromosome 11 open reading frame 30 antibody
- Emsy antibody
WB using EMSY antibody (ab123) demonstrating the interaction between EMSY and BRCA2 by immunoprecipitation.
Endogenous coimmunoprecipitation of EMSY and BRCA2 from unmanipulated asynchronously dividing HeLa cells.
Immunoprecipitation with an unrelated antibody (anti-GFP), preimmune serum, ab123 or anti-BRCA2 antibody was followed by Western blotting with ab123.
Neither of the unrelated antibodies could coprecipitate EMSY, whereas an EMSY signal was detected in the immunoprecipitate obtained with the anti-BRCA2 antibody. The anti-EMSY antibody could also immunoprecipitate endogenous EMSY protein.
EMSY was immunoprecipitated using 0.5mg MCF7 whole cell extract, Rabbit polyclonal to EMSY diluted to 1/3,000 in RIPA buffer and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab123.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 141kDa; EMSY
Western blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1/500
Lane 2: ab123 at 1/500.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Secondary ab Lane 1: Rabbit polyclonal to Goat IgG H&L (HRP) ab6741 (1/5000)
Exposure time: 3 min.
Secondary ab Lane 2: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
Exposure time: 10 sec.
Lysate at 20
Lane 1 - Exposure time : 3 min.
Lane 2 - Exposure time : 10 sec.
Variability in the size at which EMSY runs by Western blot has been exper
ab123 は 5 報の論文で使用されています。
- Kolinjivadi AM et al. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell 67:867-881.e7 (2017). PubMed: 28757209
- Altinisik J et al. Expression of EMSY gene in sporadic ovarian cancer. Mol Biol Rep 38:359-63 (2011). WB ; Human . PubMed: 20349280
- Wang Z et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal 3:ra2 (2010). PubMed: 20068230
- Seedhouse CH et al. DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412. Leukemia 20:2130-6 (2006). PubMed: 17066094
- Hughes-Davies L et al. EMSY Links the BRCA2 Pathway to Sporadic Breast and Ovarian Cancer. Cell 115:523-35 (2003). ICC/IF, IP ; Mouse . PubMed: 14651845