Anti-Emi1 抗体 [EPR15320-103] (ab187144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15320-103] to Emi1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Emi1 antibody [EPR15320-103]
Emi1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15320-103] to Emi1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, 293, HepG2 whole cell lysate (ab7900); Human ovarian carcinoma and mouse liver tissues; HeLa and HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15320-103 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF | (1) |
1/100 - 1/250.
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IP |
1/30 - 1/40.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/250. |
IP
1/30 - 1/40. |
ターゲット情報
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機能
Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. -
配列類似性
Contains 1 F-box domain.
Contains 1 IBR-type zinc finger. -
発生段階
Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis. -
ドメイン
The C-terminal region is required for inhibition of APC activity. -
翻訳後修飾
Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. - Information by UniProt
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参照データベース
- Entrez Gene: 26271 Human
- Entrez Gene: 67141 Mouse
- Omim: 606013 Human
- SwissProt: Q9UKT4 Human
- SwissProt: Q7TSG3 Mouse
- Unigene: 520506 Human
- Unigene: 197520 Mouse
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別名
- Early mitotic inhibitor 1 antibody
- Emi 1 antibody
- EMI1 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.
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All lanes : Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation of Emi1 from HepG2 cell lysate using ab187144 at 1/50 (lane 1) or 1X PBS (negative control) (lane 2). Immunostained with ab187144 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Immunofluorescent analysis of HepG2 cells labeling Emi1 with ab187144 at 1/250 and DAPI staining (blue).
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Immunofluorescent analysis of HeLa cells labeling Emi1 with ab187144 at 1/100 and DAPI staining (blue).
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Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab187144 は 1 報の論文で使用されています。
- Liu L et al. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. Biomed Res Int 2018:7849294 (2018). PubMed: 29850565