保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab49244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
機能Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
配列類似性Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
- Information by UniProt
- Elk4 antibody
- ELK4_HUMAN antibody
- ETS domain containing protein Elk4 antibody
Anti-ELK4 antibody (ab49244) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS.
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
ab49244 has not yet been referenced specifically in any publications.