Anti-Elastin 抗体 [BA-4] (ab9519)

Thank you for your enquiry.

I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml, while the Anti-Elastin antibody [BA-4] (ab9519) is estimated to be around 0.1-0.2 mg/mL.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab9519 in rat. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I am very pleased to hear you would like to accept our offer and test ab9519 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Vielen Dank für Ihren Anruf.


Ich habe einen Proteinsequenz Vergleich zwischen den Immunogenen derMaus Klone [BA-4] und[10B8] (ab9519, ab97963und ab77804)durchgeführt, und folgendes hat sich ergeben: Die Übereinstimmung zwischen dem Immunogen und demRatten Protein beträgt höchstens%. Damit ein Antikörper kreuzreagiert, sollte die Übereinstimmung mindestens 85% betragen. Es daher unwahrscheinlich, dass diese Antikörper IhrZielproteindetektieren werden.

Allerdings haben wir einen polyklonalenKaninchen Antikörper, der sowohl an der Ratte als auch in der ICC getestet wurde und dazu noch eine hohe Spezifität aufweist.

https://www.abcam.com/Elastin-antibody-ab23748.html (or use the following: https://www.abcam.com/Elastin-antibody-ab23748.html).


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Merci de votre appel et de votre intérêt pour nos anticorps contre l'élastine. Comme discuté au téléphone, nous n'avons malheureusement pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester un de nos anticorps contre l'élastine sur le poisson, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de l'anticorps acheté pourra être utilisable lors d’une prochaine commande d’anticorps primaire. Nous avons par exemple le ab9519, anticorps monoclonal de souris, testé en coupes paraffine, et sur les espèces suivantes: Hu, Cat, Dog, Goat, Gpig, Pig, Shp. Click here (or use the following: https://www.abcam.com/Elastin-antibody-BA-4-ab9519.html) ou l'anticorps polyclonal, le ab21610 testé aussi en coupes paraffine et sur les éspèces suivantes  ö Hu, Rat, Chk, Cow, Dog, Pig Click here (or use the following: https://www.abcam.com/Elastin-antibody-ab21610.html). Étapes à suivre pour participer à cette offre : 1. Nous confirmer quel anticorps vous souhaiteriez tester chez le poisson afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de cet anticorps contre l'élastine. 2. Acheter l'anticorps contre l'élastine par téléphone, fax ou internet (www.abcam.com) 3. Le tester chez le poisson 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si l'anticorps contre l'élastine est démontré ne pas fonctionner chez le poisson, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.  

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. With this specific antibody it has been shown that the method of antigen retrieval can be crucial in its performance and enzymatic retrieval is the most efficient. However, having reviewed the protocol details, I believe this product should have given satisfactory results with the methods used and unfortunately cannot find any additional suggestions to make. May I ask, did the positive control you carried out with the pericardium also give a negative result? It appears that you may have received a faulty vial of this antibody. I apologise for the inconvenience caused by this and would like to offer you a free of charge replacement of this antibody with a different lot or if this is unsatisfactory I can offer a credit note. In order to process this can you please let me know the original order number, the date on which it was placed or the address used to process it. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thanks for your reply. I'm sorry but the specific enzyme used for antigen retrieval with ab9519 is not known. I think it would be useful to try pepsin or trypsin as well. Also, what type of sample tissue are you working with?