Anti-Elastin 抗体 (ab21607)

Thank you for your inquiry.

The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

I think I found an antibody that would work for you and that is ab21600, anti-tropoelastin. This works in mouse in ICC, IHC-Fr, IHC-P, ELISA, WB, andIP.

https://www.abcam.com/Tropoelastin-antibody-ab21600.html

I hope this information helps. Please contact us with any other questions.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab21607. I would also appreciate if you can confirm some further details: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. I would try including this step. A number of other researchers on IHC world have stained Elastin successfully be using proteinase K antigen retrieval at 37°C for 20 min. This is another step I would try but without the pretreatment detailed above. How was the antibody treated prior to storage at -20°C? Glycerol should be added to a final volume of 50% prior to storage. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Thank you for your reply. I can confirm that ab21607 alpha Elastin antibody has been tested and guaranteed for IHC-P and human and mouse samples. Therefore, this would be a suitable alternative antibody.  I would like to reassure you that we monitor feedback closely on a weekly basis and are not currently concerned about the general quality of ab77804 or of this batch.  We sell quite a lot of this antibody, and regrettably, I can suggest you have received a bad vial on this occasion.  I would be pleased to arrange an free of charge replacement, which would still be covered by our Abpromise guarantee. Alternatively, I understand you concerns and if you wish to receive an alternative free of charge vial of ab21607 I would be pleased to arrange this. Please let me know if you have any further questions, and I look forward to hearing from you with details of how you would like to proceed.