Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7412-47] to eIF5A2
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
製品名Anti-eIF5A2 antibody [EPR7412-47]
eIF5A2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7412-47] to eIF5A2
特異性The antibody may react with not only A2 but also A1.
アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human eIF5A2 aa 100-200. The exact sequence is proprietary.
- HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（KD 値）KD = 2.11 x 10 -10 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab126733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
機能mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
組織特異性Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
配列類似性Belongs to the eIF-5A family.
翻訳後修飾eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
細胞内局在Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
- Information by UniProt
- 9630038B20 antibody
- eIF 5A 2 antibody
- eIF 5A2 antibody
All lanes : Anti-eIF5A2 antibody [EPR7412-47] (ab126733) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
ab126733 at 1/100 dilution, staining eIF5A2 in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab126733, at 1/100 dilution, staining eLF5A2 in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).
Equilibrium disassociation constant (KD)
Learn more about KD
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