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Anti-eIF5A2 + eIF5A 抗体 [EPR7412-50] - BSA and Azide free (ab271915)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
  • Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

製品の概要

  • 製品名

    Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free
    eIF5A2 + eIF5A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7412-50] to eIF5A2 + eIF5A - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Recombinant Human eIF5A2 protein (ab99140) can be used as a positive control in WB. HepG2, HeLa, 293T and Raji cell lysates; Human colon adenocarcinoma and testis tissues.

  • 特記事項

    ab271915 is the carrier-free version of ab150439. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Human
All applications
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IP.

    • ターゲット情報

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

      Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation (ab150439).

    • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

      Lane 1 : Recombinant Human eIF5A protein (ab87457)
      Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 17 kDa
      Observed band size: 15,20 kDa why is the actual band size different from the predicted?


      Exposure time: 7 seconds


      Blocking buffer: 5% NFDM/TBST

      This data was developed using ab150439, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation (ab150439).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

      Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation (ab150439).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

      Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation (ab150439).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

      Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation (ab150439).

    • Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)
      Anti-eIF5A2 + eIF5A antibody [EPR7412-50] - BSA and Azide free (ab271915)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271915 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271915 は論文での使用が確認できていません。

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