Anti-EIF5 抗体 (ab85913)
Key features and details
- Rabbit polyclonal to EIF5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EIF5 antibody
EIF5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
免疫原
Synthetic peptide, corresponding to a region between residues 100 and 150 of Human EIF5 (NP_892116.2)
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ポジティブ・コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85913の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
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機能
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). -
配列類似性
Belongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 423479 Chicken
- Entrez Gene: 504752 Cow
- Entrez Gene: 1983 Human
- Entrez Gene: 100047658 Mouse
- Entrez Gene: 217869 Mouse
- Entrez Gene: 56783 Rat
- Entrez Gene: 380003 Xenopus laevis
- Entrez Gene: 493571 Xenopus tropicalis
see all -
別名
- 2810011H21Rik antibody
- D12Ertd549e antibody
- EIF 5 antibody
see all
画像
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All lanes : Anti-EIF5 antibody (ab85913) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 49 kDa
Observed band size: 49 kDa
Detection: Chemiluminescence
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab85913 は 4 報の論文で使用されています。
- Loughran G et al. Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection. PLoS One 13:e0192648 (2018). PubMed: 29470543
- Tzani I et al. Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform. Open Biol 6:N/A (2016). PubMed: 27249819
- Chen H et al. Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamous cell carcinoma. J Cutan Pathol 40:651-6 (2013). PubMed: 23521708
- Loughran G et al. Stringency of start codon selection modulates autoregulation of translation initiation factor eIF5. Nucleic Acids Res : (2011). WB ; Human . PubMed: 22156057