Anti-eIF4EBP1 抗体 (ab2606)
Key features and details
- Rabbit polyclonal to eIF4EBP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-eIF4EBP1 antibody
eIF4EBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF4EBP1 -
由来種
Rabbit -
特異性
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
特記事項（精製）
Affinity purified using the immunising peptideimmobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
ターゲット情報
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機能
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
配列類似性
Belongs to the eIF4E-binding protein family. -
翻訳後修飾
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Entrez Gene: 100627508 Pig
- Entrez Gene: 116636 Rat
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- SwissProt: Q62622 Rat
see all -
別名
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
画像
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Western blot - Anti-eIF4EBP1 antibody (ab2606)Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
after exposure to high (lane 1) and low (lane 2) concentrations of leucine. Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB. -
Western blot - Anti-eIF4EBP1 antibody (ab2606)This image is a courtesy of Anonymous AbreviewAnti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 20,22,25 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
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Western blot - Anti-eIF4EBP1 antibody (ab2606)This image is a courtesy of Anonymous AbreviewAnti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 22,25 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
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Western blot - Anti-eIF4EBP1 antibody (ab2606)All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution
Lane 1 :Recombinant Human eIF4EBP1 protein (ab60007) at 0.01 µg
Lane 2 :Recombinant Human eIF4EBP1 protein (ab60007) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Exposure time: 10 seconds
参考文献 (24)
ab2606 は 24 報の論文で使用されています。
- Tian Y et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
- Wang M et al. Rapamycin suppresses angiogenesis and lymphangiogenesis in melanoma by downregulating VEGF-A/VEGFR-2 and VEGF-C/VEGFR-3 expression. Onco Targets Ther 12:4643-4654 (2019). PubMed: 31354297
- Wang Q et al. The mTOR Signal Pathway Is Overactivated in Human Lymphatic Malformations. Lymphat Res Biol N/A:N/A (2019). PubMed: 31381473
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
- Ma YF et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806
