Key features and details
- Rabbit polyclonal to eIF4EBP1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
eIF4EBP1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to eIF4EBP1
特異性This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
アプリケーション適用あり: IHC-Pmore details
種交差性交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
- RINm5F insulinoma cell lysate
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
特記事項（精製）Affinity purified using the immunising peptideimmobilized on solid support.
Our Abpromise guarantee covers the use of ab2606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
配列類似性Belongs to the eIF4E-binding protein family.
翻訳後修飾Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.
Anti-eIF4EBP1 antibody (ab2606) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 22,25 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution
Lane 1 :
Recombinant Human eIF4EBP1 protein (ab60007) at 0.01 µg
Lane 2 :
Recombinant Human eIF4EBP1 protein (ab60007) at 0.001 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
ab2606 は 24 報の論文で使用されています。
- Tian Y et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
- Wang M et al. Rapamycin suppresses angiogenesis and lymphangiogenesis in melanoma by downregulating VEGF-A/VEGFR-2 and VEGF-C/VEGFR-3 expression. Onco Targets Ther 12:4643-4654 (2019). PubMed: 31354297
- Wang Q et al. The mTOR Signal Pathway Is Overactivated in Human Lymphatic Malformations. Lymphat Res Biol N/A:N/A (2019). PubMed: 31381473
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
- Ma YF et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806