アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    eif4e-phospho-s209-antibody-ab4774.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
Share by email

Anti-eIF4E (phospho S209) 抗体 (ab4774)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)
  • Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)

Key features and details

  • Rabbit polyclonal to eIF4E (phospho S209)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Recombinant Human eIF4E protein (ab56276)

関連製品

製品の概要

  • 製品名

    Anti-eIF4E (phospho S209) antibody
    eIF4E 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to eIF4E (phospho S209)
  • 由来種

    Rabbit
  • 特異性

    This phosphorylation site specific antibody is selective for eIF-4E containing a phosphate on serine 209.
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, a wide range of other species
  • 免疫原

    Synthetic peptide (Human) derived from the region of eIF-4E that contains serine 209, based on the Homo sapiens sequence. This region is identical among many species including rat, mouse and rabbit.

  • ポジティブ・コントロール

    • HeLa cell lysate
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eIF-4E. The final product is generated by affinity chromatography using an eIF-4E-derived peptide that is phosphorylated at serine 209.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human eIF4E protein (ab56276)

アプリケーション

Our Abpromise guarantee covers the use of ab4774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能

    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 配列類似性

    Belongs to the eukaryotic initiation factor 4E family.
  • 翻訳後修飾

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Target information above from: UniProt accession P06730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1977 Human
    • Entrez Gene: 13684 Mouse
    • Entrez Gene: 117045 Rat
    • Omim: 133440 Human
    • SwissProt: P06730 Human
    • SwissProt: P63073 Mouse
    • SwissProt: P29338 Rabbit
    • SwissProt: P63074 Rat
    • Unigene: 249718 Human
    • Unigene: 3941 Mouse
    • Unigene: 11275 Rat
    see all
  • 別名

    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

画像

  • Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)
    Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)
    HeLa cell lysates (A) and HeLa cell lysates treated with alkaline phosphatase (B) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to PVDF membrane. Membranes were incubated with 1 µg/mL ab4774. After washing, membranes were incubated with goat  (ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. HeLa cell lysates (A) and HeLa cell lysates treated with alkaline phosphatase (B) were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to PVDF membrane. Membranes were incubated with 1 µg/mL ab4774. After washing, membranes were incubated with goat (ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method.
  • Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)
    Western blot - Anti-eIF4E (phospho S209) antibody (ab4774)
    Extracts of HeLa cells were resolved by SDS-PAGE on a 4-20% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, either left untreated (1-4) or treated with lambda (?) phosphatase (5), and then incubated with the eIF4E (phospho S209) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,5), the nonphosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Pierce SuperSignal™ method.

    The data show that only the peptide corresponding to eIF4E (phospho S209) blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody i

プロトコール

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (1)

    ab4774 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4774 は 1 報の論文で使用されています。

    • Kapasi P  et al. L13a blocks 48S assembly: role of a general initiation factor in mRNA-specific translational control. Mol Cell 25:113-26 (2007). PubMed: 17218275

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    What is the concentration of batch 132987?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2005

    Answer

    Thank you for your enquiry. The concentration is 0.5 mg/mL. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2005

    Question

    Here are two blots, one from abCAM and one from cell signaling! And all the details you requested! Lot Number 63956, Abcam order number: 49277, P.O. number: 3P668468. 1. Please describe the problem (high background, wrong band size, more bands, no band etc). Wrong size approximately 36kD and 65kD! 2. On what material are you testing the antibody in WB? Species? Brain tissue lyaste from Guinea Pig along with Pos and Neg cell line lyastae! Cell extract/ Nuclear extract? Whole cell lysate. Purified protein? No Recombinant protein? No 3. How much protein did you load? From 10 to 50 ug, we always run a standard curve with increasing amount of protein to evaluate an antibody of interest before doing the various experimental conditions. How did you prepare the lysate for the analysis (protease inhibito 50 mM Tris-HCl, Standard lysis buffer with protease inhibitors and phosphatase inhibitors. Did you heat the samples? Yes 4. Primary Antibody Specification (in which species was it raised against)? In Rabbit. ab4744, you have all the details! At what dilution(s) have you tested this antibody? 1ug/ml Incubation time, wash step? 2 hours in 1% non-fat dry milk solution in 20 mM TBST, ph 7.6 as suggested in the protocol supplied with the antibody. Three wash steps at room temp for 5 min each. 5. Secondary Antibody Specification (in which species was it raised against)? Goat. Anti-rabbit IgG whole molecule, HRP conjugate At what dilution(s) have you tested this antibody? 1:2500 Incubation time, wash step? 1 hr at room temp, wash step as for primary antibody. Do you know whether the problems you are experiencing come from th No 6. What detection method are you using? Chemiluminescent detection method. 7. Background bands Have you eliminated the possibility that any background bands coul Is the blocking step sufficient? (We recommend blocking the membra YES Are your washing steps sufficiently stringent? (Multiple short was YES At what size are the bands migrating? Could they be degradation pr Approx 36kD and 65kD Please provide an image of your blot (as an e-mail attachment, a f Attached. 8. Optimization attempts How many times have you tried the Western? Two times! And we do western almost everyday in our lab, check the band with your competitor on the image labeled Cell Signaling Technol on the right hand side.. Do you obtain the same results every time e.g. are background band YES What steps have you altered? YES, overnight incubation with primary antibody! 9. Did you apply positive and negative controls along with the samples? Please specify. YES, see band at 36 kD and not at approx. 25kD with abCAM. On the contrary, see band at lower than 29kD with cell signaling tehnol. product. Using abCAM antibody, we did not get band on western blot at the right molecular weight. Please do the needful.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 16 2004

    Answer

    Thank you very much for the details that you have provided, and I'm sorry to hear that you are experiencing difficulty with this antibody. As stated on the online datasheet, this particular antibody has been characterized using HeLa cell lysates and has not yet been tested on other species although it is expected to cross-react with a wide range of other species, due to sequence homology. We have not had any other complaints regarding this antibody; the problem may very well be due to the batch you received. I can offer you a replacement batch to try or a refund, please let me know which one you would prefer.

    Read More

    Abcam Scientific Support

    Answered on Aug 18 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.