Anti-eIF4E 抗体 (ab1126)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-eIF4E antibody
eIF4E 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF4E -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Cow, Human -
免疫原
Recombinant full length protein (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1126の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
配列類似性
Belongs to the eukaryotic initiation factor 4E family. -
翻訳後修飾
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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参照データベース
- Entrez Gene: 281751 Cow
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: Q9N0T5 Cow
- SwissProt: P06730 Human
- Unigene: 249718 Human
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別名
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
画像
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Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Western blot of 30µ g bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa). -
IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab1126 は 14 報の論文で使用されています。
- Batool A et al. Eukaryotic Initiation Factor 4E phosphorylation acts a switch for its binding to 4E-BP1 and mRNA cap assembly. Biochem Biophys Res Commun 527:489-495 (2020). PubMed: 32336547
- Batool A et al. Eukaryotic initiation factor 4E is a novel effector of mTORC1 signaling pathway in cross talk with Mnk1. Mol Cell Biochem 465:13-26 (2020). PubMed: 31782083
- Pitchiaya S et al. Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality. Mol Cell 74:521-533.e6 (2019). PubMed: 30952514
- Sun Y et al. Newcastle disease virus induces stable formation of bona fide stress granules to facilitate viral replication through manipulating host protein translation. FASEB J 31:1337-1353 (2017). PubMed: 28011649
- Schunter AJ et al. Phosphoproteomics of colon cancer metastasis: comparative mass spectrometric analysis of the isogenic primary and metastatic cell lines SW480 and SW620. Anal Bioanal Chem 409:1749-1763 (2017). PubMed: 27987026