Anti-EIF3C 抗体 (ab19360)
Key features and details
- Rabbit polyclonal to EIF3C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EIF3C antibody
EIF3C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF3C -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Orangutan
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免疫原
Synthetic peptide corresponding to Human EIF3C aa 850-913 (C terminal).
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ポジティブ･コントロール
- WB: HeLa and HEK293T cell lysates IP: HEK293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/15000 - 1/30000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa).
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/15000 - 1/30000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa). |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
配列類似性
Belongs to the eIF-3 subunit C family.
Contains 1 PCI domain. -
翻訳後修飾
Phosphorylated. Phosphorylation is enhanced upon serum stimulation. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8663 Human
- Omim: 603916 Human
- SwissProt: Q99613 Human
- Unigene: 567374 Human
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別名
- Cell migration inducing protein 17 antibody
- eIF 3c antibody
- eIF3 p110 antibody
see all
画像
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Western blot - Anti-EIF3C antibody (ab19360)All lanes : Anti-EIF3C antibody (ab19360) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 105 kDa
Exposure time: 3 minutesDetection: Chemiluminescence
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Immunoprecipitation - Anti-EIF3C antibody (ab19360)ab19360 immunoprecipitating EIF3C in HEK293T whole cell lysate.
Lane 1 : Rabbit anti EIF3C + HEK293T whole cell lysate
Lane 2 (+): ab19360 + HEK293T whole cell lysate.
Lane 3 (-): Control IgG instead of ab19360 in HEK293T whole cell lysate.
For western blotting, ab19360 was used at 1µg/mL.
Detection: Chemiluminescence
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab19360 は論文での使用が確認できていません。
