Anti-eIF1A 抗体 [EPR12466(B)] (ab177939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12466(B)] to eIF1A
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-eIF1A antibody [EPR12466(B)]
eIF1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12466(B)] to eIF1A -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, Jurkat, A375 and 293T cell lysates, mouse and rat brain tissue lysates; IHC-P: Human endometrial carcinoma tissue; Human kidney tissue; ICC/IF: HeLa and 293T cells. Flow Cyt (intra): HeLa cells IP: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12466(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/60.
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WB |
1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
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IP |
1/10 - 1/100.
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特記事項 |
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Flow Cyt (Intra)
1/60. |
WB
1/10000 - 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
1/50 - 1/100. |
IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
IP
1/10 - 1/100. |
ターゲット情報
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関連性
eIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene). -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 1964 Human
- Entrez Gene: 302697 Rat
- Omim: 300186 Human
- SwissProt: P47813 Human
- SwissProt: Q60872 Mouse
- SwissProt: Q8BMJ3 Mouse
- SwissProt: Q6VV72 Rat
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別名
- eIF-1A antibody
- eIF-1A X isoform antibody
- eIF-4C antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified ab177939 at 1:350 dilution (1.61 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab177939 (purified) at 1:30 dilution (2µg) immunoprecipitating eIF1A in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab177939 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab177939 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:100 dilution (5.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified ab177939 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-eIF1A antibody [EPR12466(B)] (ab177939) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
All lanes : Anti-eIF1A antibody [EPR12466(B)] (ab177939) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A375 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDaThis image was generated using the unpurified version of the product.
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Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with ab177939 at 1/50 dilution.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with ab177939 at 1/50 dilution.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling EIF1AX with ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).
This image was generated using the unpurified version of the product.
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Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using ab177939 at 1/10 dilution.
This image was generated using the unpurified version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab177939 は 6 報の論文で使用されています。
- Li Y et al. Transcriptional repression of p21 by EIF1AX promotes the proliferation of breast cancer cells. Cell Prolif 53:e12903 (2020). PubMed: 32926483
- Godfrey AK et al. Quantitative analysis of Y-Chromosome gene expression across 36 human tissues. Genome Res 30:860-873 (2020). PubMed: 32461223
- Sauls K et al. Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep 22:1913-1922 (2018). WB ; Mouse . PubMed: 29444441
- Baade I et al. Extensive Identification and In-depth Validation of Importin 13 Cargoes. Mol Cell Proteomics 17:1337-1353 (2018). PubMed: 29666159
- Thakor N et al. Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation. RNA Biol 14:553-567 (2017). PubMed: 26828225
- Sakuma T et al. Murine leukemia virus uses NXF1 for nuclear export of spliced and unspliced viral transcripts. J Virol 88:4069-82 (2014). WB ; Human . PubMed: 24478440