Application
Western blot
Sample
Human Cell lysate - nuclear (Human Dermal Microvascular Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
20 µg
Specification
Human Dermal Microvascular Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Anti Rabbit IRDye 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 200 - 150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 28 2017