Anti-EHD2 抗体 (ab23935)
Key features and details
- Goat polyclonal to EHD2
- Suitable for: Flow Cyt (Intra), ELISA, ICC/IF, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-EHD2 antibody
EHD2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to EHD2 -
由来種
Goat -
特異性
This antibody is expected to recognise EHD1 protein as well as EHD2. -
アプリケーション
適用あり: Flow Cyt (Intra), ELISA, ICC/IF, ICC, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog
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免疫原
Synthetic peptide:
C-RLVPPSKRRHKGSA
, corresponding to C terminal amino acids 529 - 542 of Human EHD2. -
ポジティブ･コントロール
- Flow Cyt (Intra): A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
Peptide ELISA. |
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 20008164
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| ICC |
Use at an assay dependent concentration.
|
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| WB |
Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ELISA
Use at an assay dependent concentration. Peptide ELISA. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 20008164 |
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.003 - 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP. -
組織特異性
Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle. -
配列類似性
Contains 1 EF-hand domain.
Contains 1 EH domain. -
ドメイン
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
細胞内局在
Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. - Information by UniProt
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参照データベース
- Entrez Gene: 538348 Cow
- Entrez Gene: 30846 Human
- Entrez Gene: 259300 Mouse
- Entrez Gene: 361512 Rat
- Omim: 605890 Human
- SwissProt: Q9NZN4 Human
- SwissProt: Q8BH64 Mouse
- SwissProt: Q4V8H8 Rat
see all -
別名
- EH domain containing 2 antibody
- EH domain containing 3 antibody
- EH domain containing protein 2 antibody
see all
画像
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Immunocytochemistry - Anti-EHD2 antibody (ab23935)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry (Intracellular) - Anti-EHD2 antibody (ab23935)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.3 µg/ml + Human lung lysate at 35 µg
Predicted band size: 61 kDa -
Immunocytochemistry - Anti-EHD2 antibody (ab23935)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-EHD2 antibody (ab23935)Lane 1 : Anti-EHD2 antibody (ab23935) at 0.1 µg/ml
Lane 2 : Anti-EHD2 antibody (ab23935) at 0.3 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 61 kDa -
Immunocytochemistry - Anti-EHD2 antibody (ab23935)ICC/IF image of ab23935 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.003 µg/ml + Human Heart lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 61 kDa
Observed band size: 61 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.1 µg/ml + Human Placenta lysate in RIPA buffer at 35 µg
Predicted band size: 61 kDa
データシートおよび資料
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Datasheet download
参考文献 (24)
ab23935 は 24 報の論文で使用されています。
- Shvets E & Mendoza-Topaz C Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology. Methods Mol Biol 2169:149-166 (2020). PubMed: 32548827
- Rathinasabapathy A et al. Expression of a Human Caveolin-1 Mutation in Mice Drives Inflammatory and Metabolic Defect-Associated Pulmonary Arterial Hypertension. Front Med (Lausanne) 7:540 (2020). PubMed: 33015095
- Fan H et al. TUSC8 inhibits the development of osteosarcoma by sponging miR-197-3p and targeting EHD2. Int J Mol Med 46:1311-1320 (2020). PubMed: 32945345
- Zhou Q et al. EHD1 impairs decidualization by regulating the Wnt4/ß-catenin signaling pathway in recurrent implantation failure. EBioMedicine 50:343-354 (2019). PubMed: 31707150
- Hetmanski JHR et al. Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration. Dev Cell 51:460-475.e10 (2019). PubMed: 31607653
