Anti-EHD1 抗体 [EPR4955] - BSA and Azide free (ab247922)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4955] to EHD1 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-EHD1 antibody [EPR4955] - BSA and Azide free
EHD1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4955] to EHD1 - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen used for this product shares 66% homology with EHD3. Cross-reactivity with this protein has not been confirmed experimentally.
-
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra), WBmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab247922 is the carrier-free version of ab109747.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4955 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247922の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
|
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa. |
ターゲット情報
-
機能
Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. -
組織特異性
Highly expressed in testis. -
配列類似性
Contains 1 EF-hand domain.
Contains 1 EH domain. -
ドメイン
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
細胞内局在
Cell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures. - Information by UniProt
-
参照データベース
- Entrez Gene: 10938 Human
- Entrez Gene: 13660 Mouse
- Entrez Gene: 293692 Rat
- Omim: 605888 Human
- SwissProt: Q9H4M9 Human
- SwissProt: Q9WVK4 Mouse
- SwissProt: Q641Z6 Rat
- Unigene: 523774 Human
see all -
別名
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
see all
画像
-
This data was developed using ab109747, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDaThis data was developed using ab109747, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab247922 は論文での使用が確認できていません。