リコンビナントRabMAb

Anti-EGR2 抗体 [EPR23228-40] - BSA and Azide free (ab270755)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
  • Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
  • Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23228-40] to EGR2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free
    EGR2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23228-40] to EGR2 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Rat
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse P0 cerebellum and Mouse thymus tissue lysates; Rat thymus and Rat brain lysates. IHC-P: Mouse cerebrum tissue; Rat cerebrum and Rat skin tissue. Flow Cyt: Mouse splenocytes treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h.

  • 特記事項

    ab270755 is the carrier-free version of ab254228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
IHC-P
Rat
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ChIP,ICC/IF,IHC-Fr or IP.

    • ターゲット情報

    • 機能

      Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.

    • 関連疾患

      Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.

    • 配列類似性

      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.

    • 翻訳後修飾

      Ubiquitinated by WWP2 leading to proteasomal degradation.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AT591 antibody
        • CMT1D antibody
        • CMT4E antibody
        • DKFZp686J1957 antibody
        • E3 SUMO-protein ligase EGR2 antibody
        • Early growth response 2 antibody
        • Early growth response protein 2 antibody
        • EGR-2 antibody
        • egr2 antibody
        • EGR2_HUMAN antibody
        • FLJ14547 antibody
        • KROX 20 Drosophila homolog antibody
        • Krox 20 homolog Drosophila antibody
        • KROX-20, Drosophila, homolog (early growth response-2) antibody
        • KROX20 antibody
        • Krox20 protein antibody
        • Zinc finger protein Krox-20 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

        Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat skin (PMID: 21514423, 21514423).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

      • Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
        Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

        Flow cytometric analysis of 2% paraformaldehyde fixed, True-Nuclear buffer-permeabilized Mouse splenocyte (treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h) cells labelling EGR2 with ab245228 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Cells were surface stained with anti-CD4 conjugated to APC-Cy7 . Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or ab245228 (Right).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

        Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat cerebrum.The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

        Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the mouse cerebrum (PMID: 30122531).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

      • Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
        Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270755 は論文での使用が確認できていません。

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