Anti-EGFR (phospho Y1148) 抗体 (ab5651)

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Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651)

    Key features and details

    • Rabbit polyclonal to EGFR (phospho Y1148)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-EGFR (phospho Y1148) antibody
      EGFR 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to EGFR (phospho Y1148)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic phosphopeptide (Human) derived from the region of EGFR that contains tyrosine 1148.

    • ポジティブ･コントロール

      • A431 cells stimulated with EGF and/or NIH3T3 cells expressing human EGFR and stimulated with EGF.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab5651 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.

    ターゲット情報

    • 機能

      Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.

    • 組織特異性

      Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

    • 関連疾患

      Lung cancer
      Inflammatory skin and bowel disease, neonatal, 2

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
      Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

    • 細胞内局在

      Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
    • Information by UniProt

    • 参照データベース

      • 別名

        • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
        • Cell growth inhibiting protein 40 antibody
        • Cell proliferation inducing protein 61 antibody
        • EGF R antibody
        • EGFR antibody
        • EGFR_HUMAN antibody
        • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
        • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
        • Epidermal growth factor receptor antibody
        • erb-b2 receptor tyrosine kinase 1 antibody
        • ERBB antibody
        • ERBB1 antibody
        • Errp antibody
        • HER1 antibody
        • mENA antibody
        • NISBD2 antibody
        • Oncogen ERBB antibody
        • PIG61 antibody
        • Proto-oncogene c-ErbB-1 antibody
        • Receptor tyrosine protein kinase ErbB 1 antibody
        • Receptor tyrosine-protein kinase ErbB-1 antibody
        • SA7 antibody
        • Species antigen 7 antibody
        • Urogastrone antibody
        • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
        • wa2 antibody
        • Wa5 antibody
        see all

      画像

      • Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651)
        Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651)
        Cell extracts prepared from NIH3T3 cells expressing EGFR and either not stimulated (-) or stimulated (+) with EGF were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose and incubated with 0.50 µg/mL ab5651 antibody, following prior incubation with: no peptide (=None), the non-phosphorylated peptide corresponding to the phosphopeptide (=EGFR [pY1148] NP), or the phosphopeptide immunogen (=EGFR [pY1148] P). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar method. The data presented here show that only the phosphopeptide corresponding to this site completely blocks the antibody signal, demonstrating the high specificity of the ab5651 antibody for this phosphorylated residue. Cell extracts prepared from NIH3T3 cells expressing EGFR and either not stimulated (-) or stimulated (+) with EGF were reso

      参考文献 (1)

      ab5651 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5651 は 1 報の論文で使用されています。

      • Liao HW  et al. PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response. J Clin Invest 125:4529-43 (2015). PubMed: 26571401

      レビューと Q&A

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      Question

      Based on your information, western blotting can detect a band of approximately 140Ka. I am a little confused since to my knowledge EGFR has the molecular weight of 170Ka. In my experiments I immunoprecipitated EGFR using anti-EGFR antibody, then detected using tyrosin phosphorylation antibody, I found the major band was about 140 Ka too. ( I used EGF as activator etc). Could you please tell why I got lower molecular weight band?

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      Answer

      Thank you for your enquiry and your interest in our products. According to Swiss-Prot database the expected molecular weight of Receptor tyrosine-protein kinase ErbB-1 is 134,200 kDa. Looking at the sequence of the aminoacide and features of the polypeptide chain, we can clearly see that there are some isoforms and also potential somatic mutations in different type of tumors and cell lines, and this can explain the differences in the expected and actual band size. We hope this information will be useful for you. If you need anything further or any help then please let us know.

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