製品の概要
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製品名Anti-EGFR antibody [ICR10]
EGFR 一次抗体 製品一覧 -
製品の詳細Rat monoclonal [ICR10] to EGFR
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由来種Rat
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アプリケーション適用あり: Inhibition Assay, Flow Cyt, ELISA, ICC/IF, IP, IHC-Fr, ICCmore details
適用なし: WB -
種交差性交差種: Mouse, Human
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免疫原
Extracellular domain of Human EGF-receptor from head and neck carcinoma
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エピトープICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.
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ポジティブ･コントロール
- FACS: A431 cells IHC: Breast Carcinoma tissue WB: HN5 cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度Ion Exchange Chromatography
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特記事項（精製）Purified IgG prepared by ion exchange chromatography.
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ポリ/モノモノクローナル
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クローン名ICR10
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ミエローマunknown
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アイソタイプIgG2a
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軽鎖の種類unknown
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研究分野
関連製品
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Affibody® Molecule
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Inhibition Assay | Use at an assay dependent concentration. See ELISA Abreview. | |
| Flow Cyt | 1/50 - 1/100. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. |
ターゲット情報
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機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
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関連疾患Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
- Information by UniProt
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参照データベース
- Entrez Gene: 1956 Human
- Entrez Gene: 13649 Mouse
- Omim: 131550 Human
- SwissProt: P00533 Human
- SwissProt: Q01279 Mouse
- Unigene: 488293 Human
- Unigene: 605083 Human
- Unigene: 420648 Mouse
see all -
別名
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
画像
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![Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.co.jp/ps/products/0/ab231/Images/EGFR-Primary-antibodies-ab231-9.jpg)
Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [ICR10] (ab231)Image courtesy of an anonymous Abreview.ab231 staining EGFR in human tumor cell line xenograft in mouse by Immunohistochemistry (Frozen sections).
Tissue was fixed in acetone. Samples incubated with ab231 at a 1/100 dilution for 1 hour at 23°C. The secondary used was an undiluted HRP conjugated goat anti-rat polyclonal. -
![Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.co.jp/ps/products/0/ab231/Images/ab231_1.jpg)
Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)Staining of A431 cells with ab231 visualised with F(ab')2 rabbit anti-rat FITC conjugated secondary. -
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.co.jp/ps/products/0/ab231/Images/ab231-OEM-060623-Im1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)ICC/IF image of ab231 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab231, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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![Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)](https://www.abcam.co.jp/ps/products/0/ab231/Images/EGFR-Primary-antibodies-ab231-3.jpg)
Inhibition Assay - Anti-EGFR antibody [ICR10] (ab231)ab231 used undiluted in inhibition assay against human recombinant protein EGFR1:Fc to inhibit binding to EGF:Biotin. The primary incubated with sample for 1 hour. The antibody inhibits the binding of biotinylated EGF to coated EGFR:Fc with an IC50 ~ 5 nM.
プロトコール
参考文献
This product has been referenced in:
- Edri R et al. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6:6500 (2015). Other ; Human . Read more (PubMed: 25799239) »
- Kirscher L et al. Doxycycline Inducible Melanogenic Vaccinia Virus as Theranostic Anti-Cancer Agent. Theranostics 5:1045-57 (2015). WB . Read more (PubMed: 26199644) »
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