Anti-EGF 抗体 (ab94898)
Key features and details
- Rabbit polyclonal to EGF
- Suitable for: ELISA, Indirect ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-EGF antibody
EGF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EGF -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, Indirect ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant Human EGF
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
Constituent: PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab94898 was purified by affinity chromatography employing immobilized Human EGF matrix -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94898の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
Indirect ELISA |
Use at an assay dependent concentration.
To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.
Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions. |
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
Indirect ELISA
Use at an assay dependent concentration. To detect IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rIL-10. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa. Detection limit is 1.5-3.0 ng, under either reducing or non-reducing conditions. |
ターゲット情報
-
機能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
組織特異性
Expressed in kidney, salivary gland, cerebrum and prostate. -
関連疾患
Hypomagnesemia 4 -
配列類似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻訳後修飾
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1950 Human
- Omim: 131530 Human
- SwissProt: P01133 Human
- Unigene: 419815 Human
-
別名
- Beta urogastrone antibody
- beta-urogastrone antibody
- EGF antibody
see all
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab94898 は論文での使用が確認できていません。