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Anti-Eg5 抗体 [EPR23276-52] - BSA and Azide free (ab270754)

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Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
  • Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23276-52] to Eg5 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free
    Eg5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23276-52] to Eg5 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human testis and tonsil tissue lysate. Mouse testis tissue lysate. Jurkat, NCI-H1975, MCF7 and 4T1 whole cell lysate. IHC-P: Human tonsil and testis tissue. Human breast cancer tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 whole cell lysate.

  • 特記事項

    ab270754 is the carrier-free version of ab254298. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
ICC/IF
Mouse
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

  • 関連疾患

    Defects in KIF11 are the cause of microcephaly with or without chorioretinopathy, lymphedema, or mental retardation (MCLMR) [MIM:152950]. An autosomal dominant disorder that involves an overlapping but variable spectrum of central nervous system and ocular developmental anomalies. Microcephaly ranges from mild to severe and is often associated with mild to moderate developmental delay and a characteristic facial phenotype with upslanting palpebral fissures, broad nose with rounded tip, long philtrum with thin upper lip, prominent chin, and prominent ears. Chorioretinopathy is the most common eye abnormality, but retinal folds, microphthalmia, and myopic and hypermetropic astigmatism have also been reported, and some individuals have no overt ocular phenotype. Congenital lymphedema, when present, is typically confined to the dorsa of the feet, and lymphoscintigraphy reveals the absence of radioactive isotope uptake from the webspaces between the toes.

  • 配列類似性

    Belongs to the kinesin-like protein family. BimC subfamily.
    Contains 1 kinesin-motor domain.

  • 翻訳後修飾

    Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
    A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

  • 細胞内局在

    Cytoplasm. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EG5 antibody
      • HKSP antibody
      • KIF11 antibody
      • KIF11_HUMAN antibody
      • Kinesin family member 11 antibody
      • Kinesin like protein 1 antibody
      • Kinesin-like protein 1 antibody
      • Kinesin-like protein KIF11 antibody
      • Kinesin-like spindle protein HKSP antibody
      • Kinesin-related motor protein Eg5 antibody
      • KNSL1 antibody
      • MCLMR antibody
      • Thyroid receptor-interacting protein 5 antibody
      • TR-interacting protein 5 antibody
      • TRIP-5 antibody
      • TRIP5 antibody
      see all

    画像

    • Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Eg5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug with ab254298 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254298 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10 ug.

      Lane 2: ab254298 IP in NIH/3T3 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254298 in NIH/3T3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

      Fresh lysates were used in this IP.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunoprecipitation - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Eg5 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab254298 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254298 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10 ug.

      Lane 2: ab254298 IP in HeLa whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254298 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      Fresh lysates were used in this IP.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Eg5 with ab254298 at 1/600 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Flow Cytometry - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Eg5 with ab254298 at 1/600 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Eg5 with ab254298 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic and spindle (arrow) staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Eg5 with ab254298 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic and spindle (arrow) staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Eg5 with ab254298 at 1/4000 (0.17µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Scattered cytoplasmic staining in human breast cancer cells is observed (PMID:29181100).
      The section was incubated with ab254298 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Eg5 with 254298 at 1/1/4000 (0.17µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes of human testis is observed.
      The section was incubated with ab254298 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Eg5 with 254298 at 1/4000 (0.17µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human tonsil is observed (PMID:25277178).
      The section was incubated with ab254298 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254298).

    • Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)
      Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (ab270754)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270754 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270754 は論文での使用が確認できていません。

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