Key features and details
- Rabbit polyclonal to EED
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Cow, Human
- Isotype: IgG
EED 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to EED
特異性ab4469 specifically recognises the four isoforms of EED following the immunoprecipitation of tagged Ezh2 from HEK293 nuclear extract. However, using HEK293 nuclear lysate ab4469 predominantly recognises non-specific bands.
アプリケーション適用あり: WB, ICC/IFmore details
種交差性交差種: Cow, Human
交差が予測される動物種: Mouse, Chicken, Xenopus laevis
Synthetic peptide corresponding to Human EED aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Recombinant EED Protein
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Predicted molecular weight: 49 kDa.|
機能Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
組織特異性Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
配列類似性Belongs to the WD repeat ESC family.
Contains 7 WD repeats.
発生段階Expression peaks at the G1/S phase boundary.
細胞内局在Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
- Information by UniProt
- eed antibody
- EED protein antibody
- EED, mouse, homolog of antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EED knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4469 was shown to recognize EED in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when EED knockout samples were examined. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Rabbit polyclonal to EED (ab4469) on U-2 OS (Human bone osteosarcoma epithelial cell line) cells. Antibody used at 1/100.
U-2 OS cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).
Secondary antibody: donkey anti-rabbit (Cy3).
Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).
ab4469 は 24 報の論文で使用されています。
- Lee JW et al. RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction-point. Nat Commun 10:1897 (2019). PubMed: 31015486
- Pandya P et al. PICOT binding to chromatin-associated EED negatively regulates cyclin D2 expression by increasing H3K27me3 at the CCND2 gene promoter. Cell Death Dis 10:685 (2019). PubMed: 31527584
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Wan L et al. Phosphorylation of EZH2 by AMPK Suppresses PRC2 Methyltransferase Activity and Oncogenic Function. Mol Cell 69:279-291.e5 (2018). PubMed: 29351847
- Anwar T et al. p38-mediated phosphorylation at T367 induces EZH2 cytoplasmic localization to promote breast cancer metastasis. Nat Commun 9:2801 (2018). PubMed: 30022044