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    eea1-antibody-early-endosome-marker-ab2900.pdf

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遺伝子ノックアウト細胞株 検証済

Anti-EEA1 抗体 - Early Endosome Marker (ab2900)

  • Datasheet
  • SDS
Reviews (38)Q&A (23)References (219)

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Immunocytochemistry - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

Key features and details

  • Rabbit polyclonal to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-EEA1 antibody - Early Endosome Marker
    EEA1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to EEA1 - Early Endosome Marker
  • 由来種

    Rabbit
  • 特異性

    Detects a band at 180kDa that represents EEA1 in Western blotting on human cell lines (corresponds to results seen in Mu et al). Also detects a band at 100kDa, we are unsure as to the identity of this band. Immunofluorescence staining of EEA1 in HeLa cells yields a punctate staining pattern consistent with the cytoplasmic distribution of endosomes.
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Dog, Human, Xenopus laevis, Chinese hamster
    交差が予測される動物種: Chicken, Hamster, Cow, Zebrafish, Rhesus monkey, Aplysia
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • ICC: HepG2 cells
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human EEA1 protein (ab112379)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2900の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (23)
Use a concentration of 1 - 5 µg/ml.
WB (12)
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 160 kDa).

Abcam recommends using milk as the blocking agent.

特記事項
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 160 kDa).

Abcam recommends using milk as the blocking agent.

ターゲット情報

  • 機能

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • 配列類似性

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • ドメイン

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 細胞内局在

    Cytoplasm. Early endosome membrane.
  • Target information above from: UniProt accession Q15075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8411 Human
    • Entrez Gene: 216238 Mouse
    • Entrez Gene: 314764 Rat
    • Omim: 605070 Human
    • SwissProt: Q15075 Human
    • SwissProt: Q8BL66 Mouse
    • Unigene: 567367 Human
    • Unigene: 210035 Mouse
    • 別名

      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    画像

    • Immunocytochemistry - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Immunocytochemistry - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

      ab2900 staining EEA1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab2900 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: EEA1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: NIH3T3 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab2900 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab2900 was shown to recognize EEA1 in wild-type HAP1 cells as signal was lost at the expected MW in EEA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and EEA1 knockout samples were subjected to SDS-PAGE. Ab2900 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
      Lane 4 : HeLa whole cell lysate
      Lane 5 : HEK-293 whole cell lysate
      Lane 6 : NIH 3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 160 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (Lanes 1-3) or 3% Milk (Lanes 4-6) before being incubated with ab2900 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

      Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Alexa Fluor anti rabbit at 1/50000 dilution

      Performed under reducing conditions.

      Predicted band size: 160 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



      Fluorescence detection of secondary antibody.
    • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

      Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of rabbit polyclonal to EEA1 (ab2900, 1/200; Alexa Fluor 488 1/200; green) and monoclonal mouse anti-β.

    • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)This image is courtesy of an anonymous Abreview

      ab2900 staining mouse L-cells by ICC/IF.  Cells were formaldehyde fixed, permeabilized in Triton X-100 and incubated with ab2900 diluted 1/1000 for 1 hour at 37°C.  A Cy2® conjugated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : HEK293 Whole Cell lysate with Human EEA1 peptide (ab14946) at 1 µg

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 160 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      Lane 1 - 2 : EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug Lane 1 : as above Lane 2 : EEA1 peptide (ab14946) at 1 ug Secondary Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution Performed under reducing conditions. Predicted band size : 160kD Observed band size : 180kD (why is the actual band size different from the predicted?)
    • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
      All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/ml

      Lane 1 : Xenopus laevis lysate
      Lane 2 : Mouse 3T3 cell lysate
      Lane 3 : Mouse brain cell lysate
      Lane 4 : Mouse liver cell lysate
      Lane 5 : Rat brain cell lysate
      Lane 6 : Rat liver cell lysate
      Lane 7 : Dog lysate
      Lane 8 : CHO cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 160 kDa
      Observed band size: 100,180 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      The Western blot shows that ab14944 reacts strongly with mouse 3T3 and CHO cell lysates. Weak cross-reactivity is seen with Xenopus, mouse brain, mouse liver, rat brain and dog lysates. The antibody does not appear to cross-react with rat liver lysate.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (219)

    ab2900 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2900 は 219 報の論文で使用されています。

    • Eapen MS  et al. Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections. Am J Physiol Lung Cell Mol Physiol 320:L158-L163 (2021). PubMed: 33174446
    • Xu M  et al. Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection. mBio 12:N/A (2021). PubMed: 33531399
    • Decotret LR  et al. Receptor-type protein tyrosine phosphatase alpha (PTPa) mediates MMP14 localization and facilitates triple-negative breast cancer cell invasion. Mol Biol Cell 32:567-578 (2021). PubMed: 33566639
    • Wang H  et al. Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks. Asian J Pharm Sci 16:120-128 (2021). PubMed: 33613735
    • Ricci-Azevedo R  et al. Microneme Proteins 1 and 4 From Toxoplasma gondii Induce IL-10 Production by Macrophages Through TLR4 Endocytosis. Front Immunol 12:655371 (2021). PubMed: 33912181
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 61 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
    Antigen retrieval step
    None
    Sample
    Aplysia Tissue sections (buccal ganglia)
    Specification
    buccal ganglia
    Permeabilization
    Yes - triton 1.5%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Francoise Geffroy

    Verified customer

    投稿 Dec 06 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Caco-2 cell)
    Specification
    Caco-2 cell
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100 in TBS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 27 2013

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 30 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Fish Cell (CHSE/F (ECACC general collection ref:000217)14))
    Permeabilization
    Yes - 0.1% Triton X100 in PBS pH 7.4
    Specification
    CHSE/F (ECACC general collection ref:000217)14)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mauricio Terebiznik

    Verified customer

    投稿 Jan 04 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa (ATCC® CCL-2™))
    Permeabilization
    Yes - 0.1% Triton X100 in PBS or 20UuM Digitonin in PBSpH 7.4
    Specification
    HeLa (ATCC® CCL-2™)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mauricio Terebiznik

    Verified customer

    投稿 Dec 21 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Quail Cell (QM7 cells (ATCC® CRL-1962™))
    Permeabilization
    Yes - 0.1% Triton X100 in PBS pH 7.4
    Specification
    QM7 cells (ATCC® CRL-1962™)
    Blocking step
    Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mauricio Terebiznik

    Verified customer

    投稿 Dec 17 2020

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer Cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Cancer Cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 16 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Acinar epithelial explants in collagen)
    Permeabilization
    Yes - TritonX100 0.025% 10 min RT
    Specification
    Acinar epithelial explants in collagen
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Clara Lubeseder-Martellato

    Verified customer

    投稿 Jun 16 2016

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Pancreas or PDAC cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Pancreas or PDAC cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 14 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neuron)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    neuron
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 30 2015

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