Anti-EDR1 / PHC1 抗体 [1F3F3] (ab175424)
Key features and details
- Mouse monoclonal [1F3F3] to EDR1 / PHC1
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-EDR1 / PHC1 antibody [1F3F3] -
製品の詳細
Mouse monoclonal [1F3F3] to EDR1 / PHC1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment corresponding to Human EDR1/ PHC1 aa 758-1004. Expressed in E. Coli.
Sequence:LKESEKPLQTGLPTGLTENQSGGPLGVDSPSAELDKKANLLKCEYCGKYA PAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEANYARVRRRG PRRSSSDIARAKIQGKCHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAG HGERDLGNPNTAPPTPELHGINPVFLSSNPSRWSVEEVYEFIASLQGCQE IAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET
Database link: P78364 -
ポジティブ・コントロール
- EDR1 / PHC1 recombinant protein; HEK293 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
1F3F3 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175424の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 106 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 106 kDa. |
Flow Cyt
1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
配列類似性
Contains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1911 Human
- Entrez Gene: 13619 Mouse
- Omim: 602978 Human
- SwissProt: P78364 Human
- SwissProt: Q64028 Mouse
- Unigene: 305985 Human
- Unigene: 6822 Mouse
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別名
- Early development regulatory protein 1 antibody
- hPH1 antibody
- mPH1 antibody
see all
画像
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All lanes : Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : EDR1/PHC1 (amino acids 758-1004)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 106 kDa -
Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution + EDR1/PHC1 recombinant protein (amino acids 758-1004).
Predicted band size: 106 kDa -
Flow cytometric analysis of HEK293 cells labeling EDR1 / PHC1 with ab175424 at 1/200 dilution (green) and negative control (red).
データシートおよび資料
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Datasheet download
参考文献 (0)
ab175424 は論文での使用が確認できていません。