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  1. Link

    ebv-nuclear-antigenebna1-antibody-e1-25-ab8329.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus EBNA
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Anti-EBV Nuclear Antigen/EBNA1 抗体 [E1-2.5] (ab8329)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (7)

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [E1-2.5] to EBV Nuclear Antigen/EBNA1
  • Suitable for: IHC-P, ICC, ELISA
  • Isotype: IgG2b

こちらの製品もご検討ください

タンパク質
Recombinant EBV Nuclear Antigen/EBNA1 protein (ab138345)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4] - BSA and Azide free (ab78113)

関連製品

製品の概要

  • 製品名

    Anti-EBV Nuclear Antigen/EBNA1 antibody [E1-2.5]
    EBV Nuclear Antigen/EBNA1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [E1-2.5] to EBV Nuclear Antigen/EBNA1
  • 由来種

    Mouse
  • 特異性

    Recognizes repetitive gly-ala region of the nuclear antigen 1 of Epstein Barr virus (EBNA-1).
  • アプリケーション

    適用あり: IHC-P, ICC, ELISAmore details
  • 種交差性

    交差種: Other species
  • 免疫原

    Synthetic peptide. Corresponding to the repetitive Gly-Ala region of EBNA1.
    Database link: P03211

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    E1-2.5
  • アイソタイプ

    IgG2b
  • 研究分野

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • EBNA

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant EBV Nuclear Antigen/EBNA1 protein (ab138345)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8329の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. PubMed: 18258188
ICC
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. PubMed: 18258188
ICC
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
  • 細胞内局在

    Nuclear. Free in the nucleoplasm, somewhat associated with the chromatin and hardly, if at all associated with the nuclear matrix.
  • 別名

    • EBNA1 antibody
    • EBV nuclear antigen 1 antibody
    • Epstein Barr nuclear antigen 1 antibody
    • Epstein Barr virus antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    see all

プロトコール

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (7)

ab8329 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8329 は 7 報の論文で使用されています。

  • Wang H  et al. The Hsp70 inhibitor 2-phenylethynesulfonamide inhibits replication and carcinogenicity of Epstein-Barr virus by inhibiting the molecular chaperone function of Hsp70. Cell Death Dis 9:734 (2018). PubMed: 29959331
  • Wang C  et al. Berberine inhibits the proliferation of human nasopharyngeal carcinoma cells via an Epstein-Barr virus nuclear antigen 1-dependent mechanism. Oncol Rep 37:2109-2120 (2017). PubMed: 28259949
  • Ni C  et al. In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures. Cell Res 25:785-800 (2015). PubMed: 25916549
  • Cardinaud S  et al. The synthesis of truncated polypeptides for immune surveillance and viral evasion. PLoS One 5:e8692 (2010). PubMed: 20098683
  • Fawzy S  et al. Detection of Epstein-Barr virus in breast carcinoma in Egyptian women. Clin Biochem 41:486-92 (2008). IHC-P . PubMed: 18258188
  • Haque T  et al. Allogeneic cytotoxic T-cell therapy for EBV-positive posttransplantation lymphoproliferative disease: results of a phase 2 multicenter clinical trial. Blood 110:1123-31 (2007). IHC-P . PubMed: 17468341
  • McClain MT  et al. An altered immune response to Epstein-Barr nuclear antigen 1 in pediatric systemic lupus erythematosus. Arthritis Rheum 54:360-8 (2006). PubMed: 16385527

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Question

David would like details of how ab8329 was raised e.g. ..."a synthetic peptide of 15 amino acids.." Also details of ab25653. What is the epitope of this antibody.

Read More

Abcam community

Verified customer

Asked on Feb 24 2006

Answer

Thank you for your enquiry. I am afraid that I have been unable to find out further details with regards the immunogen used for ab8329. I will endeavour to get back in touch with the source of this antibody, but at present they have no further information. With respect to ab25653; a recombinant GST-EBNA1 (459-641aa) was used as the antigen. Unfortunately the epitope has not been mapped for this antibody. I am sorry that I cannot be of more assistance at this point in time.

Read More

Abcam Scientific Support

Answered on Feb 24 2006

Question

Has this antibody been tested in Western blotting? Did it work or not? Do you have a publication in which this antibody was used?

Read More

Abcam community

Verified customer

Asked on Jan 17 2006

Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). We do have another EBNA1 antibody that has been tested for application in Western blotting, it is ab25653, Mouse monoclonal [1B5] to EBNA1. Please contact us again if you have any additional questions.

Read More

Abcam Scientific Support

Answered on Jan 18 2006

Question

Immunogen details of Gly-Ala repeat. Smallest immunogen used by David is 8a/as

Read More

Abcam community

Verified customer

Asked on Dec 05 2005

Answer

Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately they were unable to provide me with specific epitope details. However, details of the specificity for this antibody are such that it recognizes the repetitive gly-ala region. Examination of the Swiss-Prott entry P03211 located at the following link suggests that there is a repeating core unit of AGAGG which increases to AGAGGG after several repeats and is eventually interspersed with AGG repeats. Given that the epitope resides within this repeat region it is likely to have strong cross reactivity against different regions of the repeat region on account of the similarity of the sequence. Therefore it is highly likely that the antibody would recognise an 8 amino acid sequence that is comprised of the repeat core unit AGAGG. However, the exact sequence that the antibody recognizes has not been fully determined. http://ca.expasy.org/cgi-bin/sprot-ft-details.pl?P03211@COMPBIAS@87@352 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 06 2005

Question

I would just like to know if your secondary antibody ab6786 (goat polyclonal to mouse IgG H&L conjugated to Rhodamine) would be suitable in conjunction with antibody ab8329 (EBNA 1 antibody, mouse monoclonal IgG2b). It is not listed in your list of recommended secondaries for this antibody, but I cannot see from the data sheet why it would not work, Please advise,

Read More

Abcam community

Verified customer

Asked on Jan 21 2005

Answer

Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

Read More

Abcam Scientific Support

Answered on Jan 21 2005

Question

1. Can Abcam provide any publication supporting the ab8329,EBNA-1 monoclonal antibody ( ie can be used in immuno-staining)? 2. Can ab8329, EBNA-1 be used in both immuno-staining and immunoblotting (wester blot)? If yes, any suggested dilution factor?

Read More

Abcam community

Verified customer

Asked on Apr 19 2004

Answer

Thank you for your enquiry and your interest in our products. According to the datasheet, this antibody has been tested for ELISA and ICC applications but we do not have any information if it works for Western blot. We would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1:100-1:2000) and then adjust the dilution accordingly from the results obtained.

Read More

Abcam Scientific Support

Answered on Apr 20 2004

Question

Dear David, Hi! Please kindly advise whether anti EBNA 1 ab8329 is applicable by IHC on paraffin sections. Thanks! Best regards, Shannon Chua Marketing Manager

Read More

Abcam community

Verified customer

Asked on Dec 03 2003

Answer

This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Read More

Abcam Scientific Support

Answered on Dec 04 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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