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  1. Link

    ebp50nherf-1-antibody-ab3452.pdf

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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial
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Anti-EBP50/NHERF-1 抗体 (ab3452)

  • Datasheet
  • SDS
Reviews (8)Q&A (2)References (38)

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Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

Key features and details

  • Rabbit polyclonal to EBP50/NHERF-1
  • Suitable for: WB, IP, IHC-Fr, ICC, IHC-P
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-EBP50/NHERF-1 antibody [EPR5562] (ab109430)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-EBP50/NHERF-1 antibody
    EBP50/NHERF-1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to EBP50/NHERF-1
  • 由来種

    Rabbit
  • 特異性

    Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.
  • アプリケーション

    適用あり: WB, IP, IHC-Fr, ICC, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Dog, Human
    交差が予測される動物種: Rabbit, Cow, Cynomolgus monkey
  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: MCF7, T47D, SK-BR-3, HeLa, HepG2; Caco-2. IHC: mouse airway ICC: T47D cells
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3452の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 15703401
ICC
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
特記事項
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 15703401
ICC
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • 組織特異性

    Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • 関連疾患

    Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • 配列類似性

    Contains 2 PDZ (DHR) domains.
  • 翻訳後修飾

    Phosphorylated on serine residues.
  • 細胞内局在

    Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Target information above from: UniProt accession O14745 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 505242 Cow
    • Entrez Gene: 9368 Human
    • Entrez Gene: 26941 Mouse
    • Entrez Gene: 100009196 Rabbit
    • Entrez Gene: 59114 Rat
    • Omim: 604990 Human
    • SwissProt: Q3SZK8 Cow
    • SwissProt: O14745 Human
    • SwissProt: P70441 Mouse
    • SwissProt: Q28619 Rabbit
    • SwissProt: Q9JJ19 Rat
    • Unigene: 724482 Human
    • Unigene: 728760 Human
    • Unigene: 27842 Mouse
    • Unigene: 35142 Rat
    see all
  • 別名

    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

画像

  • Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (ab3452)
    Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (ab3452)

    Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized T-47D cells staining EBP50/NHERF-1 with ab3452 at 2µg/ml, and  Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI  (blue), and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control used no primary antibody.

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 0.2 µg/ml

    Lane 1 : Membrane enriched extracts of MCF7 (human breast adenocarcinoma cell line)
    Lane 2 : Membrane enriched extracts of T-47D (Human ductal breast epithelial tumor cell line)
    Lane 3 : Membrane enriched extracts of SK-BR-3 (Human mammary gland adenocarcinoma cell line)
    Lane 4 : Membrane enriched extracts of MDA-MB-231 (human breast adenocarcinoma cell line)
    Lane 5 : Membrane enriched extracts of HeLa (human epithelial cell line from cervix adenocarcinoma)
    Lane 6 : Membrane enriched extracts of HepG2 (human liver hepatocellular carcinoma cell line)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1 µg/ml

    Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell extract
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

    Predicted band size: 50 kDa

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from human HepG2 cells

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 40 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole tissue lysate prepared from mouse liver

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)

    Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (38)

ab3452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3452 は 38 報の論文で使用されています。

  • Capdevielle C  et al. HDAC inhibition induces expression of scaffolding proteins critical for tumor progression in pediatric glioma: focus on EBP50 and IRSp53. Neuro Oncol 22:550-562 (2020). PubMed: 31711240
  • Kammala AK  et al. Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation. Int J Mol Sci 21:N/A (2020). PubMed: 33092043
  • Leiphrakpam PD  et al. Prognostic and therapeutic implications of NHERF1 expression and regulation in colorectal cancer. J Surg Oncol 121:547-560 (2020). PubMed: 31867736
  • Wakeham CM  et al. Identification of PKCa-dependent phosphoproteins in mouse retina. J Proteomics 206:103423 (2019). PubMed: 31255707
  • Benhamouche-Trouillet S  et al. Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis. Development 145:N/A (2018). PubMed: 29712669
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (RBL-2H3)
Permeabilization
Yes - 0.5% saponin
Specification
RBL-2H3
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 09 2021

IHC - Wholemount abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (FETAL MEMBRANE)
Specification
FETAL MEMBRANE
Read More

DR. Ananth Kumar Kammala

Verified customer

投稿 Jan 29 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (AMNION EPITHELIAL CELLS)
Permeabilization
Yes - 0.3% TRITON-X-100
Specification
AMNION EPITHELIAL CELLS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 29 2021

Western blot abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Fetal membrane cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Treatment
100ng of LPS
Specification
Fetal membrane cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

DR. Ananth Kumar Kammala

Verified customer

投稿 Jan 26 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 17 2014

Western blot abreview for Anti-EBP50/NHERF-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney tissue lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Kidney tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jan 10 2011

Western blot abreview for Anti-EBP50 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
20 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Oct 30 2008

Western blot abreview for Anti-EBP50 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Oct 30 2008

Question

ab3452 (EBP50 antibody) What epitope does this antibody recognize? I need to demonstrate to a reviewer that ab3452 is not crossreactive to tubulin.

Read More

Abcam community

Verified customer

Asked on Dec 21 2006

Answer

Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the product datasheet. I hope this helps with the reviewers. Good luck with your manuscript submission. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

Answered on Dec 29 2006

Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 70213 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human cell lines HL60, Jurket and BJAB. whole cell lysates PRIMARY ANTIBODY ab3452: used at 1:1000, incubate at room temperature for 1 hour, wash 5 times and 15 minutes per wash ab9526: as above SECONDARY ANTIBODY ab3452: Goat anti rabbit IgG, 1:5000, [a competitor], incubated and washed per 1' ab9526: sheep anti mouse IgG, 1:5000, [a competitor], incubated and washed per 1' DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED ab3452: rabbit polyclonal anti-moesin [a competitor] ab9526: KPL1 which is a mouse monoclonal (IgG) to human PSGL-1 Both negative controls always gave us a band of the proper MW ANTIBODY STORAGE CONDITIONS The antibody was aliquoted and is stored at -20C. Working stock is thawed on ice and stored a 4C. SAMPLE PREPARATION 1% TX-100 lysis buffer with 4 different protease inhibitors. Samples were boiled for 2 minutes in SDS sample buffer AMOUNT OF PROTEIN LOADED WCL from 4 x 10^6 cells ELECTROPHORESIS/GEL CONDITIONS Non reduced, 7.5 % separating and 4 % stacking TRANSFER AND BLOCKING CONDITIONS Standard western transfer buffer, 1 hour, blocked with 2 % gelatin for 2 hours. MW markers transfered to Nitrocellulose (could visualize these as intense bands!) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES The comments are for both Abs I purchased from abcam. This is the second comment form I have filled out, I believe the first one was emailed on4/6/05. ab3452: gave us a very weak signal of the appropriate MW, no background bands were noted. ab9526: no appropriate signal and no background

Read More

Abcam community

Verified customer

Asked on Apr 08 2005

Answer

I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and to try more concentrated antibody (e.g 1:200-1:500). A good positive control for EBP50 is T84 cell lysate. Please do not hesitate to contact us again if you still have problems with the positive control and those recommendations,

Read More

Abcam Scientific Support

Answered on Apr 11 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
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