Anti-EBP50/NHERF-1 抗体 (ab3452)

Reviews (4)Q&A (2)References (32)

Key features and details

  • Rabbit polyclonal to EBP50/NHERF-1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-EBP50/NHERF-1 antibody
    EBP50/NHERF-1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to EBP50/NHERF-1

  • 由来種

    Rabbit

  • 特異性

    Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Dog, Human
    交差が予測される動物種: Rabbit, Cow, Cynomolgus monkey

  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.

  • 組織特異性

    Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

  • 関連疾患

    Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).

  • 配列類似性

    Contains 2 PDZ (DHR) domains.

  • 翻訳後修飾

    Phosphorylated on serine residues.

  • 細胞内局在

    Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

画像

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from human HepG2 cells

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 40 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole tissue lysate prepared from mouse liver

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)

    Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.

参考文献 (32)

ab3452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3452 は 32 報の論文で使用されています。

  • Tsurudome Y  et al. Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function. Sci Rep 8:9072 (2018). PubMed: 29899468
  • Henkel B  et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
  • Vogl C  et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep 18:2015-2029 (2017). PubMed: 28893864
  • Datta A  et al. Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cell Signal 40:91-98 (2017). PubMed: 28888686
  • Bertrand CA  et al. The CFTR trafficking mutation F508del inhibits the constitutive activity of SLC26A9. Am J Physiol Lung Cell Mol Physiol 312:L912-L925 (2017). PubMed: 28360110
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jun 17 2014

Western blot abreview for Anti-EBP50/NHERF-1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney tissue lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Kidney tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Jan 10 2011

Western blot abreview for Anti-EBP50 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
20 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Oct 30 2008

Western blot abreview for Anti-EBP50 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Oct 30 2008

Question

ab3452 (EBP50 antibody) What epitope does this antibody recognize? I need to demonstrate to a reviewer that ab3452 is not crossreactive to tubulin.

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Answer

Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the product datasheet. I hope this helps with the reviewers. Good luck with your manuscript submission. Please don't hesitate to contact us again if you have further questions.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 70213 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human cell lines HL60, Jurket and BJAB. whole cell lysates PRIMARY ANTIBODY ab3452: used at 1:1000, incubate at room temperature for 1 hour, wash 5 times and 15 minutes per wash ab9526: as above SECONDARY ANTIBODY ab3452: Goat anti rabbit IgG, 1:5000, [a competitor], incubated and washed per 1' ab9526: sheep anti mouse IgG, 1:5000, [a competitor], incubated and washed per 1' DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED ab3452: rabbit polyclonal anti-moesin [a competitor] ab9526: KPL1 which is a mouse monoclonal (IgG) to human PSGL-1 Both negative controls always gave us a band of the proper MW ANTIBODY STORAGE CONDITIONS The antibody was aliquoted and is stored at -20C. Working stock is thawed on ice and stored a 4C. SAMPLE PREPARATION 1% TX-100 lysis buffer with 4 different protease inhibitors. Samples were boiled for 2 minutes in SDS sample buffer AMOUNT OF PROTEIN LOADED WCL from 4 x 10^6 cells ELECTROPHORESIS/GEL CONDITIONS Non reduced, 7.5 % separating and 4 % stacking TRANSFER AND BLOCKING CONDITIONS Standard western transfer buffer, 1 hour, blocked with 2 % gelatin for 2 hours. MW markers transfered to Nitrocellulose (could visualize these as intense bands!) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES The comments are for both Abs I purchased from abcam. This is the second comment form I have filled out, I believe the first one was emailed on4/6/05. ab3452: gave us a very weak signal of the appropriate MW, no background bands were noted. ab9526: no appropriate signal and no background

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Answer

I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and to try more concentrated antibody (e.g 1:200-1:500). A good positive control for EBP50 is T84 cell lysate. Please do not hesitate to contact us again if you still have problems with the positive control and those recommendations,

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